Antimutagenic terpenes and sterol from chloroform extract of Malachra fasciata and Elephantopus scaber

• Main Author: Agbayani, Virgilio N.
• Format: text
• Language: English
• Published: Animo Repository 1997
• Subjects: Antimutagens; Terpenes; Sterols; Chloroform; Extracts; Herbals; Medicinal plants; Plant metabolites; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/etd_masteral/1791; https://animorepository.dlsu.edu.ph/cgi/viewcontent.cgi?article=8629&context=etd_masteral
• Institution: De La Salle University

Malachra fasciata afforded loliolide, a glycerol derivative and stigmasterol. The structures of loliolide and the glycerol derivative were elucidated using 1H-NMR, 13C-NMR, DEPT, HMQC and HMBC. Stigmasterol was identified by comparison of its 1H-NMR spectral data with those found in the literature.Five compounds were re-isolated from E. scaber: stigmasterol, lupeol lupeol acetate, deoxyelephantopin and isodeoxyelephantopin. The compounds were identified by comparing their melting points and Rf values against those found in the literature.Results of Micronucleus test showed 64.4 percent, 79.09 percent, 78.6 percent, 80.7 percent, 81.0 percent and 79.3 percent reduction in micronucleated polychromatic erythrocytes induced by Mitomycin C when loliolide, stigmasterol, lupeol acetate, lupeol, deoxyelephantopin and isodeoxyelephantopin, respectively were administered to mice of the Swiss strain. These indicate that all samples tested are antimutagens.