Screening of enterobacteriaceae among selected cold beverages sold in Quiapo, Manila

• Main Authors: Abrigo, Angela Kryztel D., Ang, Adrian Benedict E., Imbuido, Daniel B., Mendoza, Trishanne Louise T., Pandi, Charlene Annika B.
• Format: text
• Language: English
• Published: Animo Repository 2024
• Subjects: Enterobacteriaceae; Beverages > Philippines > Manila; Biology
• Online Access: https://animorepository.dlsu.edu.ph/etdb_bio/65; https://animorepository.dlsu.edu.ph/context/etdb_bio/article/1072/viewcontent/2024_Abrigo_EtAL_Screening_of_Enterobacteriaceae_Among_Selected_Cold_Beverages_Full_text.pdf
• Institution: De La Salle University

The street food and beverage scene in the Philippines has created an influx of stalls offering classic delicacies and new dishes catering to the Filipino and global palette. With this, there is also an increased risk for introducing bacterial contamination to the goods sold in these street kiosks. Foodborne pathogens have been a continuous problem, as these lead to a multitude of diseases. Enterobacteriaceae, a large family of gram-negative bacteria, are commonly isolated in food and water due to unhygienic handling and preparation. In this study, the proponents screened for the presence of Enterobacteriaceae among beverages sold in Quiapo, Manila.The samples were collected around Quiapo, Manila, specifically in QQ Mall (12 isolates), Isetann Cinerama Complex Mall (6 isolates), Quinta Market & Fish Port (15 isolates), and Plaza Miranda (7 isolates). The obtained CFU/ml of the four samples (M1 = 4.8 x 104 CFU/ml; M2 = 3.5 x 104 ; S1 = 1.7 x 105 CFU/ml; S2 = 8.05 x 104 CFU/ml) from four collection sites were deemed to be above the acceptable colony count standards provided by the Food and Drug Administration (FDA). These were screened for the presence of Enterobacteriaceae and antimicrobial resistance. A total of 40 isolates from the four samples were first identified. However, the similarities of each isolate led to the decrease in the total number of isolates, settling with 11 as the final number, which was subjected to confirmatory biochemical tests, API20E, and antimicrobial susceptibility testing. Based on the API20E results, the representative isolates were identified as Escherichia coli (2 out of 11; 18%), Enterobacter (2 out of 11; 18%), Klebsiella (2 out of 11; 18%), Kluyvera (2 out 11; 18%), Shigella (1 out of 11; 5%), Salmonella (1 out of 11; 5%), and Vibrio (1 out of 11; 5%). For the disc diffusion assay, all of the isolates showed no resistance to the following antibiotics: Chloramphenicol, Gentamycin, Tetracycline, and Ceftazidime. Five (5) out of the 11 (45%) isolates showed either intermediate or complete resistance to Amoxicillin while two (2) out of the 11 (18%) isolates showed intermediate resistance to Ertapenem. Those showing a form of resistance were retested. All of the isolates (5 out of 5; 100%) that were retested for Amoxicillin resistance showed complete resistance to the drug while all of the isolates (2 out of 2; 100%) that were retested for Ertapenem resistance were deemed to be not resistant.