In vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system

• Main Authors: Ombid, Ric John L., Oyong, Glenn G., Cabrera, Esperanza C., Espulgar, Wilfred V., Saito, Masato, Tamiya, Eiichi, Pobre, Romeric F.
• Format: text
• Published: Animo Repository 2020
• Subjects: Leukemia; Apoptosis; Cell membranes; Physics
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11545
• Institution: De La Salle University

We studied the elastic profile of monocytic THP-1 leukemia cells using a microfluidic- assisted optical trap. A 2-μm fused silica bead was optically trapped to mechanically dent an immobilized single THP-1 monocyte sieved on a 15-μm microfluidic capture chamber. Cells treated with Zeocin and untreat...