In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines

• Main Authors: Bennett, Reuel M., Dagamac, Nikki Heherson A., Fernandez, Eisha Viena M., Uba, Marigold O., Ching, Maricar
• Format: text
• Published: Animo Repository 2012
• Subjects: Polycyclic aromatic hydrocarbons; Anthracene; Mycobacterium—Philippines—Guimaras Island; Bioremediation—Philippines—Guimaras Island; Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11577
• Institution: De La Salle University

An isolate, coded as GIPAH-01, was resistant to 5 mg/LFluorene, Pyrene, Anthracene and Naphthalene. Of the four PAHs, Anthracene degradation by whole bacterial cells was assayed in vitro utilizing three levels of temperature (25, 35 and 45 ̊C), four levels of pH (5 to 8) and Anthracene concentration (1, 3 and 5 mg/L) for six days. Growth analysis at 620 nm OD following statistical analyses showed pH 7 incubated at 35 ̊C with 1 mg/LAnthracene was construed as the optimum condition for degradation analysis. Comparative growth (620 nm OD) and degradation (238 nm OD) kinetics, following optimum condition, was plotted and inferred that there was an inversely proportional interaction to which: as growth increased, Anthracene OD decreased after six days. Moreover, TLC of test Anthracene showed small developed spot with low intensity as compared with control at 235 nm OD-UVilluminator. Taxon of Anthracene degrading bacteria was identified as Mycobacterium sp. GIPAH-01 based from its morphology and biochemical properties. Results obtained in the degradation analyses of the isolate affirmed its promising biotechnological application in removing PAH in contaminated environment.