In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines

An isolate, coded as GIPAH-01, was resistant to 5 mg/LFluorene, Pyrene, Anthracene and Naphthalene. Of the four PAHs, Anthracene degradation by whole bacterial cells was assayed in vitro utilizing three levels of temperature (25, 35 and 45 ̊C), four levels of pH (5 to 8) and Anthracene concentration...

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Main Authors: Bennett, Reuel M., Dagamac, Nikki Heherson A., Fernandez, Eisha Viena M., Uba, Marigold O., Ching, Maricar
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Published: Animo Repository 2012
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/11577
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Institution: De La Salle University
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-115642024-03-25T09:40:06Z In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines Bennett, Reuel M. Dagamac, Nikki Heherson A. Fernandez, Eisha Viena M. Uba, Marigold O. Ching, Maricar An isolate, coded as GIPAH-01, was resistant to 5 mg/LFluorene, Pyrene, Anthracene and Naphthalene. Of the four PAHs, Anthracene degradation by whole bacterial cells was assayed in vitro utilizing three levels of temperature (25, 35 and 45 ̊C), four levels of pH (5 to 8) and Anthracene concentration (1, 3 and 5 mg/L) for six days. Growth analysis at 620 nm OD following statistical analyses showed pH 7 incubated at 35 ̊C with 1 mg/LAnthracene was construed as the optimum condition for degradation analysis. Comparative growth (620 nm OD) and degradation (238 nm OD) kinetics, following optimum condition, was plotted and inferred that there was an inversely proportional interaction to which: as growth increased, Anthracene OD decreased after six days. Moreover, TLC of test Anthracene showed small developed spot with low intensity as compared with control at 235 nm OD-UVilluminator. Taxon of Anthracene degrading bacteria was identified as Mycobacterium sp. GIPAH-01 based from its morphology and biochemical properties. Results obtained in the degradation analyses of the isolate affirmed its promising biotechnological application in removing PAH in contaminated environment. 2012-10-01T07:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/11577 Faculty Research Work Animo Repository Polycyclic aromatic hydrocarbons Anthracene Mycobacterium—Philippines—Guimaras Island Bioremediation—Philippines—Guimaras Island Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Polycyclic aromatic hydrocarbons
Anthracene
Mycobacterium—Philippines—Guimaras Island
Bioremediation—Philippines—Guimaras Island
Biology
spellingShingle Polycyclic aromatic hydrocarbons
Anthracene
Mycobacterium—Philippines—Guimaras Island
Bioremediation—Philippines—Guimaras Island
Biology
Bennett, Reuel M.
Dagamac, Nikki Heherson A.
Fernandez, Eisha Viena M.
Uba, Marigold O.
Ching, Maricar
In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines
description An isolate, coded as GIPAH-01, was resistant to 5 mg/LFluorene, Pyrene, Anthracene and Naphthalene. Of the four PAHs, Anthracene degradation by whole bacterial cells was assayed in vitro utilizing three levels of temperature (25, 35 and 45 ̊C), four levels of pH (5 to 8) and Anthracene concentration (1, 3 and 5 mg/L) for six days. Growth analysis at 620 nm OD following statistical analyses showed pH 7 incubated at 35 ̊C with 1 mg/LAnthracene was construed as the optimum condition for degradation analysis. Comparative growth (620 nm OD) and degradation (238 nm OD) kinetics, following optimum condition, was plotted and inferred that there was an inversely proportional interaction to which: as growth increased, Anthracene OD decreased after six days. Moreover, TLC of test Anthracene showed small developed spot with low intensity as compared with control at 235 nm OD-UVilluminator. Taxon of Anthracene degrading bacteria was identified as Mycobacterium sp. GIPAH-01 based from its morphology and biochemical properties. Results obtained in the degradation analyses of the isolate affirmed its promising biotechnological application in removing PAH in contaminated environment.
format text
author Bennett, Reuel M.
Dagamac, Nikki Heherson A.
Fernandez, Eisha Viena M.
Uba, Marigold O.
Ching, Maricar
author_facet Bennett, Reuel M.
Dagamac, Nikki Heherson A.
Fernandez, Eisha Viena M.
Uba, Marigold O.
Ching, Maricar
author_sort Bennett, Reuel M.
title In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines
title_short In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines
title_full In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines
title_fullStr In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines
title_full_unstemmed In vitro degradation of anthracene by Mycobacterium sp. GIPAH-01 isolated from Guimaras Island, Philippines
title_sort in vitro degradation of anthracene by mycobacterium sp. gipah-01 isolated from guimaras island, philippines
publisher Animo Repository
publishDate 2012
url https://animorepository.dlsu.edu.ph/faculty_research/11577
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