Optimization of culture conditions for mycelial growth and basidiocarp production of Cyclocybe cylindracea (Maire)

• Main Authors: Landingin, Harold Ray R., Francisco, Bismark E., Dulay, Rich Milton R., Kalaw, Sofronio P., Reyes, Renato G.
• Format: text
• Published: Animo Repository 2020
• Subjects: Strophariaceae; Mushroom culture
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/11449
• Institution: De La Salle University

To develop production technology to popularize the utilization of the mushroom, Cyclocybe cylindracea, the influence of culture media, pH, and physical factors such as aeration, illumination, and temperature for optimum mycelial growth and fruiting body production was investigated. Among the media evaluated, potato sucrose agar, rice bran decoction agar, and malt extract agar recorded the highest mycelial growth rate (9.00 mm/day), shortest incubation period (10.00 days) with very thick mycelial density. Potato sucrose agar (most common culture medium) at pH 6 incubated in either sealed or unsealed, lighted or dark, and in room temperature (28-33°C) or air-conditioned temperature (23-25 oC), registered the highest mean mycelial growth rate. The substrate formulation of 7:3 rice straw-sawdust ratio recorded the highest total yield of 106.94 g, and biological efficiency of 12.89%. These findings are significant in the generation of practical production technology that can be adopted by Filipino mushroom growers and those from other countries with similar environmental conditions, to increase the number of cultivated mushrooms regardless of the strain origin.