Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures

Amphidinol 3 (AM3) and theonellamide A (TNM-A) are potent antifungal compounds produced by the dinoflagellate Amphidinium klebsii and the sponge Theonella spp., respectively. Both of these metabolites have been demonstrated to interact with membrane lipids ultimately resulting in a compromised bilay...

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Main Author: Espiritu, R. A.
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Published: Animo Repository 2017
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/1636
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-26352021-07-08T03:00:59Z Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures Espiritu, R. A. Amphidinol 3 (AM3) and theonellamide A (TNM-A) are potent antifungal compounds produced by the dinoflagellate Amphidinium klebsii and the sponge Theonella spp., respectively. Both of these metabolites have been demonstrated to interact with membrane lipids ultimately resulting in a compromised bilayer integrity. In this report, the activity of AM3 and TNM-A in ternary lipid mixtures composed of 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC):brain sphingomyelin:cholesterol at a mole ratio of 1:1:1 or 3:1:1 exhibiting lipid rafts coexistence is presented. It was found that AM3 has a more extensive membrane permeabilizing activity compared with TNM-A in these membrane mimics, which was almost complete at 15 μM. The extent of their activity nevertheless is similar to the previously reported binary system of POPC and cholesterol, suggesting that phase separation has neither beneficial nor detrimental effects in their ability to disrupt the lipid bilayer. © 2017 Walter de Gruyter GmbH, Berlin/Boston. 2017-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/1636 Faculty Research Work Animo Repository Antifungal agents Lipid membranes Chemistry
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Antifungal agents
Lipid membranes
Chemistry
spellingShingle Antifungal agents
Lipid membranes
Chemistry
Espiritu, R. A.
Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures
description Amphidinol 3 (AM3) and theonellamide A (TNM-A) are potent antifungal compounds produced by the dinoflagellate Amphidinium klebsii and the sponge Theonella spp., respectively. Both of these metabolites have been demonstrated to interact with membrane lipids ultimately resulting in a compromised bilayer integrity. In this report, the activity of AM3 and TNM-A in ternary lipid mixtures composed of 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC):brain sphingomyelin:cholesterol at a mole ratio of 1:1:1 or 3:1:1 exhibiting lipid rafts coexistence is presented. It was found that AM3 has a more extensive membrane permeabilizing activity compared with TNM-A in these membrane mimics, which was almost complete at 15 μM. The extent of their activity nevertheless is similar to the previously reported binary system of POPC and cholesterol, suggesting that phase separation has neither beneficial nor detrimental effects in their ability to disrupt the lipid bilayer. © 2017 Walter de Gruyter GmbH, Berlin/Boston.
format text
author Espiritu, R. A.
author_facet Espiritu, R. A.
author_sort Espiritu, R. A.
title Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures
title_short Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures
title_full Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures
title_fullStr Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures
title_full_unstemmed Membrane permeabilizing action of amphidinol 3 and theonellamide A in raft-forming lipid mixtures
title_sort membrane permeabilizing action of amphidinol 3 and theonellamide a in raft-forming lipid mixtures
publisher Animo Repository
publishDate 2017
url https://animorepository.dlsu.edu.ph/faculty_research/1636
_version_ 1705153096350433280