Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines

Aims: The current work determined the cytotoxicity of the symbiont bioluminescent bacteria isolated from fresh ink of the squid, Photololigo duvaucelii for human colon cancer cell lines HT-29 and HCT116 and normal human dermal fibroblasts. Methodology and results: The crude sterile culture supernata...

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Main Authors: Luyon, Jocelyn Pilante, Oyong, Glenn Gallego, Cabrera, Esperanza Chua
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Published: Animo Repository 2017
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/2290
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Institution: De La Salle University
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-32892021-08-23T05:56:03Z Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines Luyon, Jocelyn Pilante Oyong, Glenn Gallego Cabrera, Esperanza Chua Aims: The current work determined the cytotoxicity of the symbiont bioluminescent bacteria isolated from fresh ink of the squid, Photololigo duvaucelii for human colon cancer cell lines HT-29 and HCT116 and normal human dermal fibroblasts. Methodology and results: The crude sterile culture supernatants of the bacterial isolates grown in marine broth containing 2.8% of NaCl were tested for their cytotoxic activities for HT-29 and HCT-116 human colon cancer cells and the normal human dermal fibroblasts HDFn using Presto Blue™ Resazurin Assay. Zeocin served as the positive control. The cytotoxicity index profiles of all culture supernatants and negative control (marine broth with 2.8% NaCl) for HDFn suggest non-toxicity to the cells, whereas most culture supernatants were observed to be cytotoxic to the two colon cancer cell lines. The culture supernatants of the isolates were found to be more cytotoxic for the HT-29 colon cancer cells than to the HCT-116 colon cancer cells. At the same time, the IC50 values showed that 85% (17/20) and 40% (8/20) of the culture supernatants tested were significantly lower, hence more potent than zeocin for HT-29 and HCT-116, respectively (p < 0.05). The rest were equally potent (p > 0.05). The 16S rRNA gene sequence analysis of the bioluminescent isolates studied revealed that they have a 97-99% similarity identity with Photobacterium leiognathi. Conclusion, significance and impact of study: This may be the first report on the cytotoxic activities on cancer cells of P. leiognathi from the Philippine squid and suggests the potential use of the secondary metabolites of these bioluminescent bacteria as anti-cancer agents. 2017-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/2290 Faculty Research Work Animo Repository Squids—Microbiology Cell-mediated cytotoxicity Metabolites Colon (Anatomy)—Cancer Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Squids—Microbiology
Cell-mediated cytotoxicity
Metabolites
Colon (Anatomy)—Cancer
Biology
spellingShingle Squids—Microbiology
Cell-mediated cytotoxicity
Metabolites
Colon (Anatomy)—Cancer
Biology
Luyon, Jocelyn Pilante
Oyong, Glenn Gallego
Cabrera, Esperanza Chua
Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines
description Aims: The current work determined the cytotoxicity of the symbiont bioluminescent bacteria isolated from fresh ink of the squid, Photololigo duvaucelii for human colon cancer cell lines HT-29 and HCT116 and normal human dermal fibroblasts. Methodology and results: The crude sterile culture supernatants of the bacterial isolates grown in marine broth containing 2.8% of NaCl were tested for their cytotoxic activities for HT-29 and HCT-116 human colon cancer cells and the normal human dermal fibroblasts HDFn using Presto Blue™ Resazurin Assay. Zeocin served as the positive control. The cytotoxicity index profiles of all culture supernatants and negative control (marine broth with 2.8% NaCl) for HDFn suggest non-toxicity to the cells, whereas most culture supernatants were observed to be cytotoxic to the two colon cancer cell lines. The culture supernatants of the isolates were found to be more cytotoxic for the HT-29 colon cancer cells than to the HCT-116 colon cancer cells. At the same time, the IC50 values showed that 85% (17/20) and 40% (8/20) of the culture supernatants tested were significantly lower, hence more potent than zeocin for HT-29 and HCT-116, respectively (p < 0.05). The rest were equally potent (p > 0.05). The 16S rRNA gene sequence analysis of the bioluminescent isolates studied revealed that they have a 97-99% similarity identity with Photobacterium leiognathi. Conclusion, significance and impact of study: This may be the first report on the cytotoxic activities on cancer cells of P. leiognathi from the Philippine squid and suggests the potential use of the secondary metabolites of these bioluminescent bacteria as anti-cancer agents.
format text
author Luyon, Jocelyn Pilante
Oyong, Glenn Gallego
Cabrera, Esperanza Chua
author_facet Luyon, Jocelyn Pilante
Oyong, Glenn Gallego
Cabrera, Esperanza Chua
author_sort Luyon, Jocelyn Pilante
title Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines
title_short Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines
title_full Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines
title_fullStr Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines
title_full_unstemmed Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines
title_sort selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of philippine squid on human colon cancer and normal cell lines
publisher Animo Repository
publishDate 2017
url https://animorepository.dlsu.edu.ph/faculty_research/2290
_version_ 1712575471506423808