Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process
Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc. Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explor...
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oai:animorepository.dlsu.edu.ph:faculty_research-38062021-11-09T01:18:08Z Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process Jacob Kizhakedathil, Moni Philip Suvarna, Suraksha Belur, Prasanna D. Wongsagonsup, Rungtiwa Agoo, Esperanza Maribel G. Janairo, Jose Isagani B. Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc. Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications. Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties. However, using oxalate oxidase can effectively remove oxalates without altering the unique properties of starch. Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology. A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied. A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal. Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions. This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase. © 2020 Taylor & Francis Group, LLC. 2020-01-01T08:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/2807 Faculty Research Work Animo Repository Oxalic acid Starch Taro Oxidoreductases Chemistry |
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Oxalic acid Starch Taro Oxidoreductases Chemistry Jacob Kizhakedathil, Moni Philip Suvarna, Suraksha Belur, Prasanna D. Wongsagonsup, Rungtiwa Agoo, Esperanza Maribel G. Janairo, Jose Isagani B. Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
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Taro (Colocasia esculenta) starch is known to possess unique physical and functional properties such as low amylose content, A-crystalline form, small granules, higher swelling power, etc. Due to the presence of significant amount of calcium oxalate crystals, the food industry is reluctant to explore this unique and cheap starch source for various food applications. Traditional processes utilizing various physical and chemical methods to remove oxalate content of starch inevitably change its physical and functional properties. However, using oxalate oxidase can effectively remove oxalates without altering the unique properties of starch. Hence, an attempt was made to optimize oxalate oxidase assisted starch extraction process from taro flour using response surface methodology. A central composite design comprising 20 experimental trials with 10 cube points augmented with six axial points and four replicates at the center point was applied. A mathematical model was developed to show the effect of taro flour concentration, enzyme load and incubation time on the oxalate removal. Validity of the model was experimentally verified and found that 98.3% of total oxalates can be removed under optimal conditions. This is the first report of optimization of the production of starch from taro flour using microbial oxalate oxidase. © 2020 Taylor & Francis Group, LLC. |
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Jacob Kizhakedathil, Moni Philip Suvarna, Suraksha Belur, Prasanna D. Wongsagonsup, Rungtiwa Agoo, Esperanza Maribel G. Janairo, Jose Isagani B. |
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Jacob Kizhakedathil, Moni Philip Suvarna, Suraksha Belur, Prasanna D. Wongsagonsup, Rungtiwa Agoo, Esperanza Maribel G. Janairo, Jose Isagani B. |
author_sort |
Jacob Kizhakedathil, Moni Philip |
title |
Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
title_short |
Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
title_full |
Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
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Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
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Optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
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optimization of oxalate-free starch production from taro flour by oxalate oxidase assisted process |
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Animo Repository |
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2020 |
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https://animorepository.dlsu.edu.ph/faculty_research/2807 |
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