In vitro cytotoxic potential of yacon (Smallanthus sonchifolius) against HT-29, MCF-7 and HDFn cell lines

• Main Authors: Mendoza, Rachelle P., Vidar, Warren S., Oyong, Glenn G.
• Format: text
• Published: Animo Repository 2018
• Subjects: Yacon; Breast—Cancer; Colon (Anatomy)—Cancer; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/3870
• Institution: De La Salle University

The objective of this study was to assess the cytotoxicity potential of hexane, methanol and DCM extracts of yacon (Smallanthus sonchifolius) leaves against breast cancer (MCF-7), colon cancer (HT-29) and normal Human Dermal Fibroblast (HDFn) cell lines by using AlamarBlue® assay. The finely ground leaves of Smallanthus sonchifolius was exhaustively extracted with hexane, followed by Dichloromethane (DCM) and lastly with methanol. All three extracts were concentrated using rotavapor and were prepared into 100 μg/mL using 0.2% Dimethyl Sulfoxide (DMSO) in complete Dulbecco's Modified Eagle Medium (DMEM) as solvent. This was then serially diluted (two-fold) during treatment on the different cell lines placed in a 96-well plate. Methotrexate, colchicine, tamoxifen and 5-fluorouracil were used as standard drug controls. AlamarBlue® was aseptically added to each well and maximum absorbance was measured at 570 nm using a microplate reader. Results showed significant reduction in cellular viability of MCF-7 cell lines caused by hexane, methanol and DCM extracts in a dose dependent manner with DCM being the most potent. The DCM extract also produced significant cytotoxic activity against HT-29 cells with the computed half maximal Inhibitory Concentration (IC) 50 significantly lower than 5-fluorouracil. Effect on HDFn showed that three yacon extracts produced significantly lower cytotoxicity compared to drug controls with the DCM extract showing the least toxicity. The extracts of leaves showed significant cytotoxic effect against breast (hexane, methanol and DCM extracts) and colon cancer cells (DCM extract) while exhibiting non-cytotoxic activities on the normal human cells compared to existing cytotoxic drugs. © Medwell Journals, 2018.