Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis

Quorum sensing (QS) refers to the phenomenon characterized by the accumulation of signaling molecules enabling a cell to sense a bacterial population triggering a coordinated response. Enterococcus faecalis is a resistant microorganism commonly found in oral infections. Salvadora persica has been tr...

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Main Authors: Rezaei, Afsaneh, Oyong, Glenn G., Borja, Virgilio B., Inoue, Masahisa, Abe, Tomomi, Tamamura, Ryo, Nagatsuka, Hitoshi, Setsu, Kojun, Buery, Rosario R.
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Published: Animo Repository 2011
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Online Access:https://animorepository.dlsu.edu.ph/faculty_research/3965
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spelling oai:animorepository.dlsu.edu.ph:faculty_research-48832021-07-26T06:40:03Z Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis Rezaei, Afsaneh Oyong, Glenn G. Borja, Virgilio B. Inoue, Masahisa Abe, Tomomi Tamamura, Ryo Nagatsuka, Hitoshi Setsu, Kojun Buery, Rosario R. Quorum sensing (QS) refers to the phenomenon characterized by the accumulation of signaling molecules enabling a cell to sense a bacterial population triggering a coordinated response. Enterococcus faecalis is a resistant microorganism commonly found in oral infections. Salvadora persica has been traditionally used in Middle East countries to clean the teeth due to its antimicrobial activity. The study investigated the anti-quorum sensing (AQS) ability of S. persica extracts on E. faecalis. Extracts from the bark, leaves, root and shoots were tested against Chromobacterium violaceum. AQS ability was determined using differential scanning fluorimetry (DSF) and elastolytic assay to detect the level of protease and quantitative polymerase chain reaction (QPCR) to detect cytolysin (cylR1) and gelatinase (gelE) virulence factors. Preliminary disk diffusion assays revealed AQS activity by inhibiting violacein pigment production in C. violaceum without affecting its growth. Furthermore, only negligible amount of proteases was detected in DSF and elastolytic assays. CylR1 and gelE gene expression revealed no detectable signals even from the lowest possible threshold. The results indicate that S. persica has AQS ability against E. faecalis. S. persica extract can be used as alternative or in combination with other anti-microbial agents against infections caused by E. faecalis. © 2010 The Hard Tissue Biology Network Association. 2011-10-20T07:00:00Z text https://animorepository.dlsu.edu.ph/faculty_research/3965 info:doi/10.2485/jhtb.20.115 Faculty Research Work Animo Repository Salvadora (Plants) Enterococcus faecalis Proteolytic enzymes Quorum sensing (Microbiology) Virulence (Microbiology) Biology
institution De La Salle University
building De La Salle University Library
continent Asia
country Philippines
Philippines
content_provider De La Salle University Library
collection DLSU Institutional Repository
topic Salvadora (Plants)
Enterococcus faecalis
Proteolytic enzymes
Quorum sensing (Microbiology)
Virulence (Microbiology)
Biology
spellingShingle Salvadora (Plants)
Enterococcus faecalis
Proteolytic enzymes
Quorum sensing (Microbiology)
Virulence (Microbiology)
Biology
Rezaei, Afsaneh
Oyong, Glenn G.
Borja, Virgilio B.
Inoue, Masahisa
Abe, Tomomi
Tamamura, Ryo
Nagatsuka, Hitoshi
Setsu, Kojun
Buery, Rosario R.
Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis
description Quorum sensing (QS) refers to the phenomenon characterized by the accumulation of signaling molecules enabling a cell to sense a bacterial population triggering a coordinated response. Enterococcus faecalis is a resistant microorganism commonly found in oral infections. Salvadora persica has been traditionally used in Middle East countries to clean the teeth due to its antimicrobial activity. The study investigated the anti-quorum sensing (AQS) ability of S. persica extracts on E. faecalis. Extracts from the bark, leaves, root and shoots were tested against Chromobacterium violaceum. AQS ability was determined using differential scanning fluorimetry (DSF) and elastolytic assay to detect the level of protease and quantitative polymerase chain reaction (QPCR) to detect cytolysin (cylR1) and gelatinase (gelE) virulence factors. Preliminary disk diffusion assays revealed AQS activity by inhibiting violacein pigment production in C. violaceum without affecting its growth. Furthermore, only negligible amount of proteases was detected in DSF and elastolytic assays. CylR1 and gelE gene expression revealed no detectable signals even from the lowest possible threshold. The results indicate that S. persica has AQS ability against E. faecalis. S. persica extract can be used as alternative or in combination with other anti-microbial agents against infections caused by E. faecalis. © 2010 The Hard Tissue Biology Network Association.
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author Rezaei, Afsaneh
Oyong, Glenn G.
Borja, Virgilio B.
Inoue, Masahisa
Abe, Tomomi
Tamamura, Ryo
Nagatsuka, Hitoshi
Setsu, Kojun
Buery, Rosario R.
author_facet Rezaei, Afsaneh
Oyong, Glenn G.
Borja, Virgilio B.
Inoue, Masahisa
Abe, Tomomi
Tamamura, Ryo
Nagatsuka, Hitoshi
Setsu, Kojun
Buery, Rosario R.
author_sort Rezaei, Afsaneh
title Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis
title_short Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis
title_full Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis
title_fullStr Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis
title_full_unstemmed Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis
title_sort molecular screening of anti-quorum sensing capability of salvadora persica on enterococcus faecalis
publisher Animo Repository
publishDate 2011
url https://animorepository.dlsu.edu.ph/faculty_research/3965
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