Radioprotection of intestinal crypt cells by cox-inhibitors
The regulation of tissue homeostasis in the gastrointestinal epithelium after epithelial injury focuses on the prostaglandins (PGs) as its major mediators. The two cyclooxygenase isoforms, Cox-1 and Cox-2, catalyze synthesis of PGs. Cox-1 is the predominant cyclooxygenase isoform found in the normal...
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Main Authors: | , , , , , |
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Format: | text |
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Animo Repository
2006
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Online Access: | https://animorepository.dlsu.edu.ph/faculty_research/5295 |
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Institution: | De La Salle University |
Summary: | The regulation of tissue homeostasis in the gastrointestinal epithelium after epithelial injury focuses on the prostaglandins (PGs) as its major mediators. The two cyclooxygenase isoforms, Cox-1 and Cox-2, catalyze synthesis of PGs. Cox-1 is the predominant cyclooxygenase isoform found in the normal intestine. In contrast, Cox-2 is present at low levels in normal intestine but is elevated at sites of inflammation, and in adenomas and carcinomas. To study the effects of various commercially-available cox-inhibitors (Ketorolac: Cox-1 selective; Celecoxib: Cox-2 selective; and Indocid: Cox-1/2 non-selective), we determine mouse crypt epithelial cell fate after genotoxic injury with whole-body gamma-ray exposure at 15 Gy. Intestinal tissues of mice treated with Cox-2 inhibitors that showed invariable apoptotic event, however, have increased occurrence of regenerating cells. Our results suggest a potential application of Cox-2 selective inhibitors as radioprotective agent for normal cells after radiotherapy. |
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