Radioprotection of intestinal crypt cells by cox-inhibitors

• Main Authors: Bisnar, Paul O., Dones, Rosa Angela S.A., Serna, Paulene-Ver A., Deocaris, Chester C., Guttierez, Kalangitan V., Deocaris, Custer C.
• Format: text
• Published: Animo Repository 2006
• Subjects: Cyclooxygenase 2—Inhibitors; Epithelial cells; Prostaglandins; Apoptosis; Biology; Cell and Developmental Biology
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/5295
• Institution: De La Salle University

The regulation of tissue homeostasis in the gastrointestinal epithelium after epithelial injury focuses on the prostaglandins (PGs) as its major mediators. The two cyclooxygenase isoforms, Cox-1 and Cox-2, catalyze synthesis of PGs. Cox-1 is the predominant cyclooxygenase isoform found in the normal intestine. In contrast, Cox-2 is present at low levels in normal intestine but is elevated at sites of inflammation, and in adenomas and carcinomas. To study the effects of various commercially-available cox-inhibitors (Ketorolac: Cox-1 selective; Celecoxib: Cox-2 selective; and Indocid: Cox-1/2 non-selective), we determine mouse crypt epithelial cell fate after genotoxic injury with whole-body gamma-ray exposure at 15 Gy. Intestinal tissues of mice treated with Cox-2 inhibitors that showed invariable apoptotic event, however, have increased occurrence of regenerating cells. Our results suggest a potential application of Cox-2 selective inhibitors as radioprotective agent for normal cells after radiotherapy.