Collagen extract from chicken skin as clot actovator

• Main Authors: Dizon, Maria Carmen S., Balatbat, Maricel S.
• Format: text
• Published: Animo Repository 2022
• Subjects: Collagen; Blood—Coagulation; Chickens; Hemic and Lymphatic Diseases
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/5995
• Institution: De La Salle University

This research goals to determine the ability of collagen extracted from chicken skin as a clot activator. It sought to identify the (1) duration of the collagen extract to induce optimum blood clot formation; (2) significant difference between each concentration of the test; and, (3) most effective concentration as an in vitro clot activator. The collagen was extracted bases on Pacak’s improved method for the preparation of type I collagen from skin and then identified by Van Gieson’s stain. This was then tested on 3 patients at 3 different concentrations 1:30, 1:40 and 1:50 in a triplicate manner. Results show that blood treated with collagen at 1:40 has a lower standard deviation (1.92188) from the control and has fastest clotting time (20.2957) among the thee. On the other hand, Tukey’s post hoc test proved that concentrations at 1:40 and 1:50 both showed effectivity. With this, the researchers reject the null hypotheses, concluding that (1) the clot formation takes shorter time than the control; (2) there is a significant difference among the different concentrations thereby, the higher the concentration; and, (3) among all the different volume of blood used, 4mL of blood, has the most potent activity in inducing the clotting activity.