Automatic Identification of the Stress Sources of Protein Aggregates Using Flow Imaging Microscopy Images

• Main Authors: Gambe-Gilbuena, Arni G, Shibano, Yuriko, Krayukhina, Elena, Torisu, Tetsuo, Uchiyama, Susumu
• Format: text
• Published: Archīum Ateneo 2019
• Subjects: analysis; biopharmaceutical characterization; protein aggregation; antibody drugs; machine learning; neural network; injectables; Biology; Biotechnology; Pharmacology
• Online Access: https://archium.ateneo.edu/biology-faculty-pubs/28; https://www.sciencedirect.com/science/article/pii/S0022354919306719
• Institution: Ateneo De Manila University

A novel approach to identify 5 types of simulated stresses that induce protein aggregation in prefilled syringe–type biopharmaceuticals was developed. Principal components analyses of texture metrics extracted from flow imaging microscopy images were used to define subgroups of particles. Supervised...