Structural analysis of κ-carrageenan sulfated oligosaccharides by positive mode Nano-ESI-FTICR-MS and MS/MS by SORI-CID

• Main Authors: Aguilan, Jennifer T, Dayrit, Fabian M, Zhang, Jinhua, Niñonuevo, Milady R, Lebrilla, Carlito B
• Format: text
• Published: Archīum Ateneo 2006
• Subjects: Chemistry; Organic Chemistry
• Online Access: https://archium.ateneo.edu/chemistry-faculty-pubs/113; https://link.springer.com/article/10.1016/j.jasms.2005.09.009
• Institution: Ateneo De Manila University

Structural analysis of sulfated oligosaccharides from κ-carrageenan of up to ten residues (MW >2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the B- and Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues.