Saliva “Treat-and-Heat” Triplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for SARS-CoV-2

• Main Authors: Reolo, Marie Jennifer Y, Eleazar, Carlos Sebastian R, Sonio, Joseph P, Solon, Ryonne T, Villamor, Janika L B, Loedin, Alexandra K, Moore, Keith J M
• Format: text
• Published: Archīum Ateneo 2021
• Subjects: fluorescence; melt curve; extraction-free; Proteinase K; Analytical, Diagnostic and Therapeutic Techniques and Equipment; Chemistry
• Online Access: https://archium.ateneo.edu/chemistry-faculty-pubs/186; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8730512/
• Institution: Ateneo De Manila University

The demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) molecular diagnostics that are faster, cheaper, and simpler to run than nasopharyngeal-based reverse transcription quantitative PCR (RT-qPCR) tests remains unmet in many parts of the world. In the Philippines, geographical a...