Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R2 ≥ 0.987)....

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Main Authors: Wang, Lin, Zhao, Lei, Mo, Yu, Tan, Swee Ngin, Wang, Jing-Yuan, Tan, Amy Giin-Yu, Chen, Chia-Lung, Ge, Liya, Li, Ling
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/102050
http://hdl.handle.net/10220/19363
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1020502020-09-26T21:58:57Z Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis Wang, Lin Zhao, Lei Mo, Yu Tan, Swee Ngin Wang, Jing-Yuan Tan, Amy Giin-Yu Chen, Chia-Lung Ge, Liya Li, Ling School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute DRNTU::Engineering::Civil engineering A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R2 ≥ 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers. Accepted version 2014-05-19T02:24:15Z 2019-12-06T20:48:48Z 2014-05-19T02:24:15Z 2019-12-06T20:48:48Z 2013 2013 Journal Article Tan, A. G.-Y., Chen, C.-L., Ge, L., Li, L., Wang, L., Zhao, L. et al. (2014). Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis. Journal of Bioscience and Bioengineering, 117(3), 379-382. 1389-1723 https://hdl.handle.net/10356/102050 http://hdl.handle.net/10220/19363 10.1016/j.jbiosc.2013.08.020 174978 en Journal of Bioscience and Bioengineering © 2013 The Society for Biotechnology, Japan. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Bioscience and Bioengineering, The Society for Biotechnology, Japan. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:http://dx.doi.org/10.1016/j.jbiosc.2013.08.020]. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Engineering::Civil engineering
spellingShingle DRNTU::Engineering::Civil engineering
Wang, Lin
Zhao, Lei
Mo, Yu
Tan, Swee Ngin
Wang, Jing-Yuan
Tan, Amy Giin-Yu
Chen, Chia-Lung
Ge, Liya
Li, Ling
Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
description A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R2 ≥ 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Wang, Lin
Zhao, Lei
Mo, Yu
Tan, Swee Ngin
Wang, Jing-Yuan
Tan, Amy Giin-Yu
Chen, Chia-Lung
Ge, Liya
Li, Ling
format Article
author Wang, Lin
Zhao, Lei
Mo, Yu
Tan, Swee Ngin
Wang, Jing-Yuan
Tan, Amy Giin-Yu
Chen, Chia-Lung
Ge, Liya
Li, Ling
author_sort Wang, Lin
title Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
title_short Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
title_full Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
title_fullStr Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
title_full_unstemmed Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
title_sort enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis
publishDate 2014
url https://hdl.handle.net/10356/102050
http://hdl.handle.net/10220/19363
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