PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation

Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated...

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Main Authors: Rotman, Nicolas, Guex, Nicolas, Gouranton, Erwan, Wahli, Walter
Other Authors: Knott, Jason Glenn
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/102143
http://hdl.handle.net/10220/18832
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1021432022-02-16T16:27:23Z PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation Rotman, Nicolas Guex, Nicolas Gouranton, Erwan Wahli, Walter Knott, Jason Glenn Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated with active genes (H3K4me3) and with silent genes (H3K27me3) atypically co-occupy chromatin regions surrounding the promoters of important developmental genes. However, it is unclear how these epigenetic marks are recognized when cell differentiation starts and what precise role they play. Here, we report the essential role of the nuclear receptor peroxisome proliferator-activated receptor β (PPARβ, NR1C2) in Xenopus laevis early development. By combining loss-of-function approaches, large throughput transcript expression analysis by the mean of RNA-seq and intensive chromatin immunoprecipitation experiments, we unveil an important cooperation between epigenetic marks and PPARβ. During Xenopus laevis gastrulation PPARβ recognizes H3K27me3 marks that have been deposited earlier at the pluripotent stage to activate early differentiation genes. Thus, PPARβis the first identified transcription factor that interprets an epigenetic signature of pluripotency, in vivo, during embryonic development. This work paves the way for a better mechanistic understanding of how the activation of hundreds of genes is coordinated during early development. Published version 2014-02-19T02:53:27Z 2019-12-06T20:50:17Z 2014-02-19T02:53:27Z 2019-12-06T20:50:17Z 2013 2013 Journal Article Rotman, N., Guex, N., Gouranton, E., & Wahli, W. (2013). PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation. PLoS ONE, 8(12), e83300-. 1932-6203 https://hdl.handle.net/10356/102143 http://hdl.handle.net/10220/18832 10.1371/journal.pone.0083300 24367589 en PLoS ONE © 2013 Rotman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Medicine
spellingShingle DRNTU::Science::Medicine
Rotman, Nicolas
Guex, Nicolas
Gouranton, Erwan
Wahli, Walter
PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
description Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated with active genes (H3K4me3) and with silent genes (H3K27me3) atypically co-occupy chromatin regions surrounding the promoters of important developmental genes. However, it is unclear how these epigenetic marks are recognized when cell differentiation starts and what precise role they play. Here, we report the essential role of the nuclear receptor peroxisome proliferator-activated receptor β (PPARβ, NR1C2) in Xenopus laevis early development. By combining loss-of-function approaches, large throughput transcript expression analysis by the mean of RNA-seq and intensive chromatin immunoprecipitation experiments, we unveil an important cooperation between epigenetic marks and PPARβ. During Xenopus laevis gastrulation PPARβ recognizes H3K27me3 marks that have been deposited earlier at the pluripotent stage to activate early differentiation genes. Thus, PPARβis the first identified transcription factor that interprets an epigenetic signature of pluripotency, in vivo, during embryonic development. This work paves the way for a better mechanistic understanding of how the activation of hundreds of genes is coordinated during early development.
author2 Knott, Jason Glenn
author_facet Knott, Jason Glenn
Rotman, Nicolas
Guex, Nicolas
Gouranton, Erwan
Wahli, Walter
format Article
author Rotman, Nicolas
Guex, Nicolas
Gouranton, Erwan
Wahli, Walter
author_sort Rotman, Nicolas
title PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
title_short PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
title_full PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
title_fullStr PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
title_full_unstemmed PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
title_sort pparβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
publishDate 2014
url https://hdl.handle.net/10356/102143
http://hdl.handle.net/10220/18832
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