PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation
Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated...
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sg-ntu-dr.10356-1021432022-02-16T16:27:23Z PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation Rotman, Nicolas Guex, Nicolas Gouranton, Erwan Wahli, Walter Knott, Jason Glenn Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated with active genes (H3K4me3) and with silent genes (H3K27me3) atypically co-occupy chromatin regions surrounding the promoters of important developmental genes. However, it is unclear how these epigenetic marks are recognized when cell differentiation starts and what precise role they play. Here, we report the essential role of the nuclear receptor peroxisome proliferator-activated receptor β (PPARβ, NR1C2) in Xenopus laevis early development. By combining loss-of-function approaches, large throughput transcript expression analysis by the mean of RNA-seq and intensive chromatin immunoprecipitation experiments, we unveil an important cooperation between epigenetic marks and PPARβ. During Xenopus laevis gastrulation PPARβ recognizes H3K27me3 marks that have been deposited earlier at the pluripotent stage to activate early differentiation genes. Thus, PPARβis the first identified transcription factor that interprets an epigenetic signature of pluripotency, in vivo, during embryonic development. This work paves the way for a better mechanistic understanding of how the activation of hundreds of genes is coordinated during early development. Published version 2014-02-19T02:53:27Z 2019-12-06T20:50:17Z 2014-02-19T02:53:27Z 2019-12-06T20:50:17Z 2013 2013 Journal Article Rotman, N., Guex, N., Gouranton, E., & Wahli, W. (2013). PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation. PLoS ONE, 8(12), e83300-. 1932-6203 https://hdl.handle.net/10356/102143 http://hdl.handle.net/10220/18832 10.1371/journal.pone.0083300 24367589 en PLoS ONE © 2013 Rotman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
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DRNTU::Science::Medicine Rotman, Nicolas Guex, Nicolas Gouranton, Erwan Wahli, Walter PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
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Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated with active genes (H3K4me3) and with silent genes (H3K27me3) atypically co-occupy chromatin regions surrounding the promoters of important developmental genes. However, it is unclear how these epigenetic marks are recognized when cell differentiation starts and what precise role they play. Here, we report the essential role of the nuclear receptor peroxisome proliferator-activated receptor β (PPARβ, NR1C2) in Xenopus laevis early development. By combining loss-of-function approaches, large throughput transcript expression analysis by the mean of RNA-seq and intensive chromatin immunoprecipitation experiments, we unveil an important cooperation between epigenetic marks and PPARβ. During Xenopus laevis gastrulation PPARβ recognizes H3K27me3 marks that have been deposited earlier at the pluripotent stage to activate early differentiation genes. Thus, PPARβis the first identified transcription factor that interprets an epigenetic signature of pluripotency, in vivo, during embryonic development. This work paves the way for a better mechanistic understanding of how the activation of hundreds of genes is coordinated during early development. |
author2 |
Knott, Jason Glenn |
author_facet |
Knott, Jason Glenn Rotman, Nicolas Guex, Nicolas Gouranton, Erwan Wahli, Walter |
format |
Article |
author |
Rotman, Nicolas Guex, Nicolas Gouranton, Erwan Wahli, Walter |
author_sort |
Rotman, Nicolas |
title |
PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
title_short |
PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
title_full |
PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
title_fullStr |
PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
title_full_unstemmed |
PPARβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
title_sort |
pparβ interprets a chromatin signature of pluripotency to promote embryonic differentiation at gastrulation |
publishDate |
2014 |
url |
https://hdl.handle.net/10356/102143 http://hdl.handle.net/10220/18832 |
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1725985549354795008 |