Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments

Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclid...

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Bibliographic Details
Main Authors: Yildiz, Umit Hakan, De Hoog, Hans-Peter M., Fu, Zhikang, Tomczak, Nikodem, Parikh, Atul N., Nallani, Madhavan, Liedberg, Bo
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2014
Subjects:
DRNTU::Engineering::Materials
Online Access:https://hdl.handle.net/10356/102791
http://hdl.handle.net/10220/19139
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Institution: Nanyang Technological University
Language: English
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Summary:Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

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