Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments

• Main Authors: Yildiz, Umit Hakan, De Hoog, Hans-Peter M., Fu, Zhikang, Tomczak, Nikodem, Parikh, Atul N., Nallani, Madhavan, Liedberg, Bo
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Materials
• Online Access: https://hdl.handle.net/10356/102791; http://hdl.handle.net/10220/19139
• Institution: Nanyang Technological University

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.