Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells

This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful b...

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Main Authors: Chan, Jerry Kok Yen, Cao, Xue, Kwek, Kenneth, Chan, Casey K. H., Lim, Mayasari
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/103015
http://hdl.handle.net/10220/19151
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1030152020-03-07T11:35:36Z Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells Chan, Jerry Kok Yen Cao, Xue Kwek, Kenneth Chan, Casey K. H. Lim, Mayasari School of Chemical and Biomedical Engineering DRNTU::Science::Chemistry This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell–cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale. 2014-04-07T05:12:25Z 2019-12-06T21:03:59Z 2014-04-07T05:12:25Z 2019-12-06T21:03:59Z 2013 2013 Journal Article Cao, X., Kwek, K., Chan, J. K. Y., Chan, C. K. H., & Lim, M. (2014). Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells. Journal of Biomedical Materials Research Part A, 102(2), 523-531. 1549-3296 https://hdl.handle.net/10356/103015 http://hdl.handle.net/10220/19151 10.1002/jbm.a.34716 en Journal of biomedical materials research part A © 2013 Wiley Periodicals, Inc.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Chemistry
spellingShingle DRNTU::Science::Chemistry
Chan, Jerry Kok Yen
Cao, Xue
Kwek, Kenneth
Chan, Casey K. H.
Lim, Mayasari
Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
description This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell–cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Chan, Jerry Kok Yen
Cao, Xue
Kwek, Kenneth
Chan, Casey K. H.
Lim, Mayasari
format Article
author Chan, Jerry Kok Yen
Cao, Xue
Kwek, Kenneth
Chan, Casey K. H.
Lim, Mayasari
author_sort Chan, Jerry Kok Yen
title Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
title_short Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
title_full Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
title_fullStr Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
title_full_unstemmed Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
title_sort electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
publishDate 2014
url https://hdl.handle.net/10356/103015
http://hdl.handle.net/10220/19151
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