Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence

Staphylococcus saprophyticus is the only species of Staphylococcus that is typically uropathogenic and possesses a gene coding for a D-serine-deaminase (DsdA). As D-serine is prevalent in urine and toxic or bacteriostatic to many bacteria, it is not surprising that the D-serine-deaminase gene is fou...

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Main Authors: Kline, Kimberly A., Korte-Berwanger, Miriam, Sakinc, Türkan, Nielsen, Hailyn V., Hultgren, Scott, Gatermann, Sören G.
Other Authors: Camilli, A.
Format: Article
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/103049
http://hdl.handle.net/10220/25761
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spelling sg-ntu-dr.10356-1030492022-02-16T16:28:01Z Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence Kline, Kimberly A. Korte-Berwanger, Miriam Sakinc, Türkan Nielsen, Hailyn V. Hultgren, Scott Gatermann, Sören G. Camilli, A. Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Immunology Staphylococcus saprophyticus is the only species of Staphylococcus that is typically uropathogenic and possesses a gene coding for a D-serine-deaminase (DsdA). As D-serine is prevalent in urine and toxic or bacteriostatic to many bacteria, it is not surprising that the D-serine-deaminase gene is found in the genome of uropathogens. It has been suggested that D-serine-deaminase or the ability to respond to or to metabolize D-serine is important for virulence. For uropathogenic Escherichia coli (UPEC), a high intracellular D-serine concentration affects expression of virulence factors. S. saprophyticus is able to grow in the presence of high D-serine concentrations; however, its D-serine metabolism has not been described. The activity of the D-serine-deaminase was verified by analyzing the formation of pyruvate from D-serine in different strains with and without D-serine-deaminase. Cocultivation experiments were performed to show that D-serine-deaminase confers a growth advantage to S. saprophyticus in the presence of D-serine. Furthermore, in vivo coinfection experiments showed a disadvantage for the ΔdsdA mutant during urinary tract infection. Expression analysis of known virulence factors by reverse transcription-quantitative PCR (RT-qPCR) showed that the surface-associated lipase Ssp is upregulated in the presence of D-serine. In addition, we show that S. saprophyticus is able to use D-serine as the sole carbon source, but interestingly, D-serine had a negative effect on growth when glucose was also present. Taken together, D-serine metabolism is associated with virulence in S. saprophyticus, as at least one known virulence factor is upregulated in the presence of D-serine and a ΔdsdA mutant was attenuated in virulence murine model of urinary tract infection. Published version 2015-06-04T08:44:26Z 2019-12-06T21:04:27Z 2015-06-04T08:44:26Z 2019-12-06T21:04:27Z 2013 2013 Journal Article Korte-Berwanger, M., Sakinc, T., Kline, K., Nielsen, H. V., Hultgren, S., & Gatermann, S. G. (2013). Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence. Infection and immunity, 81(12), 4525-4533. https://hdl.handle.net/10356/103049 http://hdl.handle.net/10220/25761 10.1128/IAI.00599-13 24082071 en Infection and immunity © 2013 American Society for Microbiology (ASM). This paper was published in Infection and Immunity and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The paper can be found at the following official DOI: [http://dx.doi.org/10.1128/IAI.00599-13]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 9 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Immunology
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Immunology
Kline, Kimberly A.
Korte-Berwanger, Miriam
Sakinc, Türkan
Nielsen, Hailyn V.
Hultgren, Scott
Gatermann, Sören G.
Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence
description Staphylococcus saprophyticus is the only species of Staphylococcus that is typically uropathogenic and possesses a gene coding for a D-serine-deaminase (DsdA). As D-serine is prevalent in urine and toxic or bacteriostatic to many bacteria, it is not surprising that the D-serine-deaminase gene is found in the genome of uropathogens. It has been suggested that D-serine-deaminase or the ability to respond to or to metabolize D-serine is important for virulence. For uropathogenic Escherichia coli (UPEC), a high intracellular D-serine concentration affects expression of virulence factors. S. saprophyticus is able to grow in the presence of high D-serine concentrations; however, its D-serine metabolism has not been described. The activity of the D-serine-deaminase was verified by analyzing the formation of pyruvate from D-serine in different strains with and without D-serine-deaminase. Cocultivation experiments were performed to show that D-serine-deaminase confers a growth advantage to S. saprophyticus in the presence of D-serine. Furthermore, in vivo coinfection experiments showed a disadvantage for the ΔdsdA mutant during urinary tract infection. Expression analysis of known virulence factors by reverse transcription-quantitative PCR (RT-qPCR) showed that the surface-associated lipase Ssp is upregulated in the presence of D-serine. In addition, we show that S. saprophyticus is able to use D-serine as the sole carbon source, but interestingly, D-serine had a negative effect on growth when glucose was also present. Taken together, D-serine metabolism is associated with virulence in S. saprophyticus, as at least one known virulence factor is upregulated in the presence of D-serine and a ΔdsdA mutant was attenuated in virulence murine model of urinary tract infection.
author2 Camilli, A.
author_facet Camilli, A.
Kline, Kimberly A.
Korte-Berwanger, Miriam
Sakinc, Türkan
Nielsen, Hailyn V.
Hultgren, Scott
Gatermann, Sören G.
format Article
author Kline, Kimberly A.
Korte-Berwanger, Miriam
Sakinc, Türkan
Nielsen, Hailyn V.
Hultgren, Scott
Gatermann, Sören G.
author_sort Kline, Kimberly A.
title Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence
title_short Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence
title_full Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence
title_fullStr Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence
title_full_unstemmed Significance of the d-Serine-deaminase and d-Serine metabolism of staphylococcus saprophyticus for virulence
title_sort significance of the d-serine-deaminase and d-serine metabolism of staphylococcus saprophyticus for virulence
publishDate 2015
url https://hdl.handle.net/10356/103049
http://hdl.handle.net/10220/25761
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