Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches

Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches

Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus by zygotic injection. Driving our efforts was...

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Bibliographic Details
Main Authors: Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2019
Subjects:
DRNTU::Science::Biological sciences
Blastocyst
CRISPR
Online Access:https://hdl.handle.net/10356/103397
http://hdl.handle.net/10220/47312
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Institution: Nanyang Technological University
Language: English

Internet

https://hdl.handle.net/10356/103397
http://hdl.handle.net/10220/47312

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