Viable mice with extensive gene humanization (25-kbp) created using embryonic stem cell/blastocyst and CRISPR/zygote injection approaches

• Main Authors: Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2019
• 主題: DRNTU::Science::Biological sciences; Blastocyst; CRISPR
• 在線閱讀: https://hdl.handle.net/10356/103397; http://hdl.handle.net/10220/47312

Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus by zygotic injection. Driving our efforts was...