Structure and function of the smoothened extracellular domain in vertebrate hedgehog signaling

• Main Authors: Rohatgi, Rajat, Nachtergaele, Sigrid, Whalen, Daniel M, Mydock, Laurel K, Zhao, Zhonghua, Malinauskas, Tomas, Krishnan, Kathiresan, Covey, Douglas F, Siebold, Christian, Ingham, Philip William
• Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Medicine
• Online Access: https://hdl.handle.net/10356/103764; http://hdl.handle.net/10220/19293
• Institution: Nanyang Technological University

The Hedgehog (Hh) signal is transduced across the membrane by the heptahelical protein Smoothened (Smo), a developmental regulator, oncoprotein and drug target in oncology. We present the 2.3 Å crystal structure of the extracellular cysteine rich domain (CRD) of vertebrate Smo and show that it bi...