Bioorthogonal cleavage and exchange of major histocompatibility complex ligands by employing azobenzene-containing peptides

• Main Authors: Choo, Joanna A. L., Thong, Sock Yue, Yap, Jiawei, van Esch, Wim J. E., Raida, Manfred, Meijers, Rob, Lescar, Julien, Verhelst, Steven H. L., Grotenbreg, Gijsbert M.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/103938; http://hdl.handle.net/10220/24627
• Institution: Nanyang Technological University

Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene-containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high-throughput studies of disease-specific T cell responses.