Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx
Chronic hepatitis B virus (HBV) carriers may develop hepatocellular carcinoma (HCC) by a wide range of mechanisms including angiogenesis. We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). Interestingly, ibuprofen (a Cox2 inhib...
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sg-ntu-dr.10356-1039842020-03-07T11:40:22Z Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx Zhang, Jianhua Chen, William Wei Ning School of Chemical and Biomedical Engineering DRNTU::Science::Medicine Chronic hepatitis B virus (HBV) carriers may develop hepatocellular carcinoma (HCC) by a wide range of mechanisms including angiogenesis. We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). Interestingly, ibuprofen (a Cox2 inhibitor) is found to attenuate the levels of IL6 and Cox 2 which are induced by HBV replication.The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. Our results show that HBx is involved in the increase of the expression of Cox2 through the NFκB pathway. However, the expression of Cox2 is decreased when the HBx-expressing cells are incubated with ibuprofen. The contrasting effect of HBx on Cox2 is found to be determined by differential dimer formation among the members of the NFκB family of proteins, including NFκB, RelA, and C-rel. Specifically, HBx alone results in dimer formation between NFκB and RelA, while the combined presence of HBx and ibuprofen leads to the formation of NFκB and C-rel. Additional information on the interaction network involving HBx, ibuprofen, and NFκB pathways is revealed by two-dimensional liquid chromatography-tandem mass spectrometry proteomics analysis. Taken together, our findings provide new insights on the angiogenesis induced by HBV replication. 2013-11-01T01:11:28Z 2019-12-06T21:23:56Z 2013-11-01T01:11:28Z 2019-12-06T21:23:56Z 2012 2012 Journal Article Zhang, J., & Chen, W. N. W. (2012). Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx. Interventional medicine and applied science, 4(1), 21-31. https://hdl.handle.net/10356/103984 http://hdl.handle.net/10220/17190 10.1556/IMAS.4.2012.1.5 en Interventional medicine and applied science |
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DRNTU::Science::Medicine Zhang, Jianhua Chen, William Wei Ning Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx |
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Chronic hepatitis B virus (HBV) carriers may develop hepatocellular carcinoma (HCC) by a wide range of mechanisms including angiogenesis. We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). Interestingly, ibuprofen (a Cox2 inhibitor) is found to attenuate the levels of IL6 and Cox 2 which are induced by HBV replication.The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. Our results show that HBx is involved in the increase of the expression of Cox2 through the NFκB pathway. However, the expression of Cox2 is decreased when the HBx-expressing cells are incubated with ibuprofen. The contrasting effect of HBx on Cox2 is found to be determined by differential dimer formation among the members of the NFκB family of proteins, including NFκB, RelA, and C-rel. Specifically, HBx alone results in dimer formation between NFκB and RelA, while the combined presence of HBx and ibuprofen leads to the formation of NFκB and C-rel. Additional information on the interaction network involving HBx, ibuprofen, and NFκB pathways is revealed by two-dimensional liquid chromatography-tandem mass spectrometry proteomics analysis. Taken together, our findings provide new insights on the angiogenesis induced by HBV replication. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Zhang, Jianhua Chen, William Wei Ning |
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Article |
author |
Zhang, Jianhua Chen, William Wei Ning |
author_sort |
Zhang, Jianhua |
title |
Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx |
title_short |
Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx |
title_full |
Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx |
title_fullStr |
Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx |
title_full_unstemmed |
Inhibition of HBV-induced angiogenesis by ibuprofen : role of HBx |
title_sort |
inhibition of hbv-induced angiogenesis by ibuprofen : role of hbx |
publishDate |
2013 |
url |
https://hdl.handle.net/10356/103984 http://hdl.handle.net/10220/17190 |
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1681048476187623424 |