Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients

• Main Authors: Kaur, Palvinder, Khong, Wei Xin, Wee, Sue Yuen, Tan, Eng Lee, Pipper, Juergen, Koay, Evelyn, Ng, Kah Ying, Yap, Joe Kwan, Chew, Kuan Kiat, Tan, Mei Ting, Leo, Yee Sin, Inoue, Masafumi, Ng, Oon Tek
• Other Authors: Kumar, Anil
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Medicine
• Online Access: https://hdl.handle.net/10356/104049; http://hdl.handle.net/10220/19482
• Institution: Nanyang Technological University

Objectives HIV-1 viral quantitation is essential for treatment monitoring. An in-house assay would decrease financial barriers to access. Materials and Methods A real-time competitive RT-PCR in house assay (Sing-IH) was developed in Singapore. Using HXB2 as reference, the assay's pri...