R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias
Disruption of the Setx gene, defective in ataxia oculomotor apraxia type 2 (AOA2) leads to the accumulation of DNA/RNA hybrids (R-loops), failure of meiotic recombination and infertility in mice. We report here the presence of R-loops in the testes from other autosomal recessive ataxia mouse models,...
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sg-ntu-dr.10356-1041142022-02-16T16:27:57Z R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias Yeo, Abrey J. Becherel, Olivier J. Luff, John E. Cullen, Jason K. Wongsurawat, Thidathip Jenjaroenpoon, Piroon Kuznetsov, Vladimir A. McKinnon, Peter J. Lavin, Martin F. Gueven, Nuri School of Computer Engineering DRNTU::Engineering::Computer science and engineering Disruption of the Setx gene, defective in ataxia oculomotor apraxia type 2 (AOA2) leads to the accumulation of DNA/RNA hybrids (R-loops), failure of meiotic recombination and infertility in mice. We report here the presence of R-loops in the testes from other autosomal recessive ataxia mouse models, which correlate with fertility in these disorders. R-loops were coincident in cells showing high basal levels of DNA double strand breaks and in those cells undergoing apoptosis. Depletion of Setx led to high basal levels of R-loops and these were enhanced further by DNA damage both in vitro and in vivo in tissues with proliferating cells. There was no evidence for accumulation of R-loops in the brains of mice where Setx, Atm, Tdp1 or Aptx genes were disrupted. These data provide further evidence for genome destabilization as a consequence of disrupted transcription in the presence of DNA double strand breaks arising during DNA replication or recombination. They also suggest that R-loop accumulation does not contribute to the neurodegenerative phenotype in these autosomal recessive ataxias. Published version 2014-06-03T03:27:19Z 2019-12-06T21:26:45Z 2014-06-03T03:27:19Z 2019-12-06T21:26:45Z 2014 2014 Journal Article Yeo, A. J., Becherel, O. J., Luff, J. E., Cullen, J. K., Wongsurawat, T., Jenjaroenpoon, P., et al. (2014). R-Loops in Proliferating Cells but Not in the Brain: Implications for AOA2 and Other Autosomal Recessive Ataxias. PLoS ONE, 9(3), e90219-. 1932-6203 https://hdl.handle.net/10356/104114 http://hdl.handle.net/10220/19519 10.1371/journal.pone.0090219 24637776 en PLoS ONE © 2014 Yeo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
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DRNTU::Engineering::Computer science and engineering Yeo, Abrey J. Becherel, Olivier J. Luff, John E. Cullen, Jason K. Wongsurawat, Thidathip Jenjaroenpoon, Piroon Kuznetsov, Vladimir A. McKinnon, Peter J. Lavin, Martin F. R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias |
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Disruption of the Setx gene, defective in ataxia oculomotor apraxia type 2 (AOA2) leads to the accumulation of DNA/RNA hybrids (R-loops), failure of meiotic recombination and infertility in mice. We report here the presence of R-loops in the testes from other autosomal recessive ataxia mouse models, which correlate with fertility in these disorders. R-loops were coincident in cells showing high basal levels of DNA double strand breaks and in those cells undergoing apoptosis. Depletion of Setx led to high basal levels of R-loops and these were enhanced further by DNA damage both in vitro and in vivo in tissues with proliferating cells. There was no evidence for accumulation of R-loops in the brains of mice where Setx, Atm, Tdp1 or Aptx genes were disrupted. These data provide further evidence for genome destabilization as a consequence of disrupted transcription in the presence of DNA double strand breaks arising during DNA replication or recombination. They also suggest that R-loop accumulation does not contribute to the neurodegenerative phenotype in these autosomal recessive ataxias. |
author2 |
Gueven, Nuri |
author_facet |
Gueven, Nuri Yeo, Abrey J. Becherel, Olivier J. Luff, John E. Cullen, Jason K. Wongsurawat, Thidathip Jenjaroenpoon, Piroon Kuznetsov, Vladimir A. McKinnon, Peter J. Lavin, Martin F. |
format |
Article |
author |
Yeo, Abrey J. Becherel, Olivier J. Luff, John E. Cullen, Jason K. Wongsurawat, Thidathip Jenjaroenpoon, Piroon Kuznetsov, Vladimir A. McKinnon, Peter J. Lavin, Martin F. |
author_sort |
Yeo, Abrey J. |
title |
R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias |
title_short |
R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias |
title_full |
R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias |
title_fullStr |
R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias |
title_full_unstemmed |
R-Loops in proliferating cells but not in the brain : implications for AOA2 and other autosomal recessive ataxias |
title_sort |
r-loops in proliferating cells but not in the brain : implications for aoa2 and other autosomal recessive ataxias |
publishDate |
2014 |
url |
https://hdl.handle.net/10356/104114 http://hdl.handle.net/10220/19519 |
_version_ |
1725985621493678080 |