Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells
Endothelial cell–matrix interactions play a vital role in promoting vascularization of engineered tissues. The current study reports a facile and controllable method to develop a RGD peptide-functionalized chitosan microsphere scaffolds for rapid cell expansion of human umbilical vein endothelial ce...
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sg-ntu-dr.10356-1041582020-06-01T10:01:40Z Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells Pehkonen, Simo O. Yang, Zhenyi Yuan, Shaojun Liang, Bin Liu, Yang Choong, Cleo Swee Neo School of Materials Science & Engineering DRNTU::Science::Medicine::Tissue engineering Endothelial cell–matrix interactions play a vital role in promoting vascularization of engineered tissues. The current study reports a facile and controllable method to develop a RGD peptide-functionalized chitosan microsphere scaffolds for rapid cell expansion of human umbilical vein endothelial cells (HUVECs). Functional poly(methacrylic acid) (PMAA) brushes are grafted from the chitosan microsphere surfaces via surface-initiated ATRP. Subsequent conjugation of RGD peptides on the pendent carboxyl groups of PMAA side chain is accomplished by carbodiimide chemistry to facilitate biocompatibility of the 3D CS scaffolding system. In vitro cell-loading assay of HUVECs exhibits a significant improvment of cell adhesion, spreading, and proliferation on the RGD peptide-immobilized CS microsphere surfaces. 2014-06-20T08:48:44Z 2019-12-06T21:27:36Z 2014-06-20T08:48:44Z 2019-12-06T21:27:36Z 2014 2014 Journal Article Yang, Z., Yuan, S., Liang, B., Liu, Y., Choong, C., & Pehkonen, S. O. (2014). Chitosan Microsphere Scaffold Tethered with RGD-Conjugated Poly(methacrylic acid) Brushes as Effective Carriers for the Endothelial Cells. Macromolecular Bioscience, in press. 1616-5187 https://hdl.handle.net/10356/104158 http://hdl.handle.net/10220/19856 10.1002/mabi.201400136 179873 en Macromolecular bioscience © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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DRNTU::Science::Medicine::Tissue engineering Pehkonen, Simo O. Yang, Zhenyi Yuan, Shaojun Liang, Bin Liu, Yang Choong, Cleo Swee Neo Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
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Endothelial cell–matrix interactions play a vital role in promoting vascularization of engineered tissues. The current study reports a facile and controllable method to develop a RGD peptide-functionalized chitosan microsphere scaffolds for rapid cell expansion of human umbilical vein endothelial cells (HUVECs). Functional poly(methacrylic acid) (PMAA) brushes are grafted from the chitosan microsphere surfaces via surface-initiated ATRP. Subsequent conjugation of RGD peptides on the pendent carboxyl groups of PMAA side chain is accomplished by carbodiimide chemistry to facilitate biocompatibility of the 3D CS scaffolding system. In vitro cell-loading assay of HUVECs exhibits a significant improvment of cell adhesion, spreading, and proliferation on the RGD peptide-immobilized CS microsphere surfaces. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Pehkonen, Simo O. Yang, Zhenyi Yuan, Shaojun Liang, Bin Liu, Yang Choong, Cleo Swee Neo |
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Article |
author |
Pehkonen, Simo O. Yang, Zhenyi Yuan, Shaojun Liang, Bin Liu, Yang Choong, Cleo Swee Neo |
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Pehkonen, Simo O. |
title |
Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
title_short |
Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
title_full |
Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
title_fullStr |
Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
title_full_unstemmed |
Chitosan microsphere scaffold tethered with RGD-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
title_sort |
chitosan microsphere scaffold tethered with rgd-conjugated poly(methacrylic acid) brushes as effective carriers for the endothelial cells |
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2014 |
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https://hdl.handle.net/10356/104158 http://hdl.handle.net/10220/19856 |
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1681057797941231616 |