In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

• Main Authors: Huang, Rong, Ferhan, Abdul Rahim, Guo, Longhua, Qiu, Bin, Lin, Zhenyu, Kim, Dong-Hwan, Chen, Guonan
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: https://hdl.handle.net/10356/105886; http://hdl.handle.net/10220/20971
• Institution: Nanyang Technological University

We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage...