In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

In situ synthesis of protein-resistant poly(oligo(ethylene glycol)methacrylate) films in capillary for protein separation

We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage...

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Bibliographic Details
Main Authors: Huang, Rong, Ferhan, Abdul Rahim, Guo, Longhua, Qiu, Bin, Lin, Zhenyu, Kim, Dong-Hwan, Chen, Guonan
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2014
Subjects:
DRNTU::Engineering::Bioengineering
Online Access:https://hdl.handle.net/10356/105886
http://hdl.handle.net/10220/20971
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Institution: Nanyang Technological University
Language: English
Description
Summary:We report a method to modify a silica capillary with poly(oligo(ethylene glycol)methacrylate) (POEGMA) and its performance for protein separation. We optimized the grafting density and thickness of the POEGMA film and investigated the effect of running buffer pH, ionic strength, separation voltage, and sample injection time. The POEGMA-coated capillary was successfully utilized for the separation of six model proteins. Under optimized conditions, the resolution was found to be much better than a bare capillary, and even better than a widely used, commercially available PEG-modified capillary.

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