Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip

• Main Authors: Wu, Bo, Chen, Li-Chan, Huang, Youju, Zhang, Yiming, Kang, Yuejun, Kim, Dong-Hwan
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Engineering::Chemical engineering::Biochemical engineering
• Online Access: https://hdl.handle.net/10356/106187; http://hdl.handle.net/10220/24981; http://dx.doi.org/10.1007/s11468-013-9661-4
• Institution: Nanyang Technological University

A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane (PDMS) layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin).