Brain site-specific proteome changes in aging-related dementia

This study is aimed at gaining insights into the brain site-specific proteomic senescence signature while comparing physiologically aged brains with aging-related dementia brains (for example, Alzheimer’s disease (AD)). Our study of proteomic differences within the hippocampus (Hp), parietal cortex...

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Main Authors: Manavalan, Arulmani, Mishra, Manisha, Feng, Lin, Sze, Siu Kwan, Akatsu, Hiroyasu, Heese, Klaus
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/106286
http://hdl.handle.net/10220/23994
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1062862023-02-28T17:07:01Z Brain site-specific proteome changes in aging-related dementia Manavalan, Arulmani Mishra, Manisha Feng, Lin Sze, Siu Kwan Akatsu, Hiroyasu Heese, Klaus School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology This study is aimed at gaining insights into the brain site-specific proteomic senescence signature while comparing physiologically aged brains with aging-related dementia brains (for example, Alzheimer’s disease (AD)). Our study of proteomic differences within the hippocampus (Hp), parietal cortex (pCx) and cerebellum (Cb) could provide conceptual insights into the molecular mechanisms involved in aging-related neurodegeneration. Using an isobaric tag for relative and absolute quantitation (iTRAQ)-based two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) brain site-specific proteomic strategy, we identified 950 proteins in the Hp, pCx and Cb of AD brains. Of these proteins, 31 were significantly altered. Most of the differentially regulated proteins are involved in molecular transport, nervous system development, synaptic plasticity and apoptosis. Particularly, proteins such as Gelsolin (GSN), Tenascin-R (TNR) and AHNAK could potentially act as novel biomarkers of aging-related neurodegeneration. Importantly, our Ingenuity Pathway Analysis (IPA)-based network analysis further revealed ubiquitin C (UBC) as a pivotal protein to interact with diverse AD-associated pathophysiological molecular factors and suggests the reduced ubiquitin proteasome degradation system (UPS) as one of the causative factors of AD. Published version 2014-10-10T07:31:58Z 2019-12-06T22:08:07Z 2014-10-10T07:31:58Z 2019-12-06T22:08:07Z 2013 2013 Journal Article Manavalan, A., Mishra, M., Feng, L., Sze, S. K., Akatsu, H., & Heese, K. (2013). Brain site-specific proteome changes in aging-related dementia. Experimental & molecular Medicine, 45(9), e39-. 2092-6413 https://hdl.handle.net/10356/106286 http://hdl.handle.net/10220/23994 10.1038/emm.2013.76 24008896 en Experimental & molecular medicine © 2013 KSBMB. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/. 17 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Manavalan, Arulmani
Mishra, Manisha
Feng, Lin
Sze, Siu Kwan
Akatsu, Hiroyasu
Heese, Klaus
Brain site-specific proteome changes in aging-related dementia
description This study is aimed at gaining insights into the brain site-specific proteomic senescence signature while comparing physiologically aged brains with aging-related dementia brains (for example, Alzheimer’s disease (AD)). Our study of proteomic differences within the hippocampus (Hp), parietal cortex (pCx) and cerebellum (Cb) could provide conceptual insights into the molecular mechanisms involved in aging-related neurodegeneration. Using an isobaric tag for relative and absolute quantitation (iTRAQ)-based two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) brain site-specific proteomic strategy, we identified 950 proteins in the Hp, pCx and Cb of AD brains. Of these proteins, 31 were significantly altered. Most of the differentially regulated proteins are involved in molecular transport, nervous system development, synaptic plasticity and apoptosis. Particularly, proteins such as Gelsolin (GSN), Tenascin-R (TNR) and AHNAK could potentially act as novel biomarkers of aging-related neurodegeneration. Importantly, our Ingenuity Pathway Analysis (IPA)-based network analysis further revealed ubiquitin C (UBC) as a pivotal protein to interact with diverse AD-associated pathophysiological molecular factors and suggests the reduced ubiquitin proteasome degradation system (UPS) as one of the causative factors of AD.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Manavalan, Arulmani
Mishra, Manisha
Feng, Lin
Sze, Siu Kwan
Akatsu, Hiroyasu
Heese, Klaus
format Article
author Manavalan, Arulmani
Mishra, Manisha
Feng, Lin
Sze, Siu Kwan
Akatsu, Hiroyasu
Heese, Klaus
author_sort Manavalan, Arulmani
title Brain site-specific proteome changes in aging-related dementia
title_short Brain site-specific proteome changes in aging-related dementia
title_full Brain site-specific proteome changes in aging-related dementia
title_fullStr Brain site-specific proteome changes in aging-related dementia
title_full_unstemmed Brain site-specific proteome changes in aging-related dementia
title_sort brain site-specific proteome changes in aging-related dementia
publishDate 2014
url https://hdl.handle.net/10356/106286
http://hdl.handle.net/10220/23994
_version_ 1759853186241265664