Brain site-specific proteome changes in aging-related dementia
This study is aimed at gaining insights into the brain site-specific proteomic senescence signature while comparing physiologically aged brains with aging-related dementia brains (for example, Alzheimer’s disease (AD)). Our study of proteomic differences within the hippocampus (Hp), parietal cortex...
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sg-ntu-dr.10356-1062862023-02-28T17:07:01Z Brain site-specific proteome changes in aging-related dementia Manavalan, Arulmani Mishra, Manisha Feng, Lin Sze, Siu Kwan Akatsu, Hiroyasu Heese, Klaus School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology This study is aimed at gaining insights into the brain site-specific proteomic senescence signature while comparing physiologically aged brains with aging-related dementia brains (for example, Alzheimer’s disease (AD)). Our study of proteomic differences within the hippocampus (Hp), parietal cortex (pCx) and cerebellum (Cb) could provide conceptual insights into the molecular mechanisms involved in aging-related neurodegeneration. Using an isobaric tag for relative and absolute quantitation (iTRAQ)-based two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) brain site-specific proteomic strategy, we identified 950 proteins in the Hp, pCx and Cb of AD brains. Of these proteins, 31 were significantly altered. Most of the differentially regulated proteins are involved in molecular transport, nervous system development, synaptic plasticity and apoptosis. Particularly, proteins such as Gelsolin (GSN), Tenascin-R (TNR) and AHNAK could potentially act as novel biomarkers of aging-related neurodegeneration. Importantly, our Ingenuity Pathway Analysis (IPA)-based network analysis further revealed ubiquitin C (UBC) as a pivotal protein to interact with diverse AD-associated pathophysiological molecular factors and suggests the reduced ubiquitin proteasome degradation system (UPS) as one of the causative factors of AD. Published version 2014-10-10T07:31:58Z 2019-12-06T22:08:07Z 2014-10-10T07:31:58Z 2019-12-06T22:08:07Z 2013 2013 Journal Article Manavalan, A., Mishra, M., Feng, L., Sze, S. K., Akatsu, H., & Heese, K. (2013). Brain site-specific proteome changes in aging-related dementia. Experimental & molecular Medicine, 45(9), e39-. 2092-6413 https://hdl.handle.net/10356/106286 http://hdl.handle.net/10220/23994 10.1038/emm.2013.76 24008896 en Experimental & molecular medicine © 2013 KSBMB. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/. 17 p. application/pdf |
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DRNTU::Science::Biological sciences::Molecular biology Manavalan, Arulmani Mishra, Manisha Feng, Lin Sze, Siu Kwan Akatsu, Hiroyasu Heese, Klaus Brain site-specific proteome changes in aging-related dementia |
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This study is aimed at gaining insights into the brain site-specific proteomic senescence signature while comparing physiologically aged brains with aging-related dementia brains (for example, Alzheimer’s disease (AD)). Our study of proteomic differences within the hippocampus (Hp), parietal cortex (pCx) and cerebellum (Cb) could provide conceptual insights into the molecular mechanisms involved in aging-related neurodegeneration. Using an isobaric tag for relative and absolute quantitation (iTRAQ)-based two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC-MS/MS) brain site-specific proteomic strategy, we identified 950 proteins in the Hp, pCx and Cb of AD brains. Of these proteins, 31 were significantly altered. Most of the differentially regulated proteins are involved in molecular transport, nervous system development, synaptic plasticity and apoptosis. Particularly, proteins such as Gelsolin (GSN), Tenascin-R (TNR) and AHNAK could potentially act as novel biomarkers of aging-related neurodegeneration. Importantly, our Ingenuity Pathway Analysis (IPA)-based network analysis further revealed ubiquitin C (UBC) as a pivotal protein to interact with diverse AD-associated pathophysiological molecular factors and suggests the reduced ubiquitin proteasome degradation system (UPS) as one of the causative factors of AD. |
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School of Biological Sciences |
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School of Biological Sciences Manavalan, Arulmani Mishra, Manisha Feng, Lin Sze, Siu Kwan Akatsu, Hiroyasu Heese, Klaus |
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Article |
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Manavalan, Arulmani Mishra, Manisha Feng, Lin Sze, Siu Kwan Akatsu, Hiroyasu Heese, Klaus |
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Manavalan, Arulmani |
title |
Brain site-specific proteome changes in aging-related dementia |
title_short |
Brain site-specific proteome changes in aging-related dementia |
title_full |
Brain site-specific proteome changes in aging-related dementia |
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Brain site-specific proteome changes in aging-related dementia |
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Brain site-specific proteome changes in aging-related dementia |
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brain site-specific proteome changes in aging-related dementia |
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2014 |
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https://hdl.handle.net/10356/106286 http://hdl.handle.net/10220/23994 |
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