Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells
We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness...
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sg-ntu-dr.10356-1062902023-07-14T15:58:18Z Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells Yu, Haiyang Lui, Yuan Siang Xiong, Sijing Leong, Wen Shing Wen, Feng Nurkahfianto, Himawan Rana, Sravendra Leong, David Tai Ng, Kee Woei Tan, Lay Poh School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering. Published version 2014-10-10T06:56:45Z 2019-12-06T22:08:13Z 2014-10-10T06:56:45Z 2019-12-06T22:08:13Z 2013 2013 Journal Article Yu, H., Lui, Y. S., Xiong, S., Leong, W. S., Wen, F., Nurkahfianto, H., et al. (2013). Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells. Stem cells and development, 22(1), 136-147. 1547-3287 https://hdl.handle.net/10356/106290 http://hdl.handle.net/10220/23990 10.1089/scd.2012.0160 22765653 en Stem cells and development © 2013 Mary Ann Liebert, Inc. This paper was published in Stem Cells and Development and is made available as an electronic reprint (preprint) with permission of Mary Ann Liebert, Inc. The paper can be found at the following official DOI: http://dx.doi.org/10.1089/scd.2012.0160. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 12 p. application/pdf |
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DRNTU::Engineering::Materials::Biomaterials Yu, Haiyang Lui, Yuan Siang Xiong, Sijing Leong, Wen Shing Wen, Feng Nurkahfianto, Himawan Rana, Sravendra Leong, David Tai Ng, Kee Woei Tan, Lay Poh Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
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We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Yu, Haiyang Lui, Yuan Siang Xiong, Sijing Leong, Wen Shing Wen, Feng Nurkahfianto, Himawan Rana, Sravendra Leong, David Tai Ng, Kee Woei Tan, Lay Poh |
format |
Article |
author |
Yu, Haiyang Lui, Yuan Siang Xiong, Sijing Leong, Wen Shing Wen, Feng Nurkahfianto, Himawan Rana, Sravendra Leong, David Tai Ng, Kee Woei Tan, Lay Poh |
author_sort |
Yu, Haiyang |
title |
Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
title_short |
Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
title_full |
Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
title_fullStr |
Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
title_full_unstemmed |
Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
title_sort |
insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells |
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2014 |
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https://hdl.handle.net/10356/106290 http://hdl.handle.net/10220/23990 |
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1773551357111828480 |