Simultaneous enrichment of plasma soluble and extracellular vesicular glycoproteins using prolonged ultracentrifugation-electrostatic repulsion-hydrophilic interaction chromatography (PUC-ERLIC) approach

• Main Authors: Cheow, Esther Sok Hwee, Sim, Kae Hwan, Lee, Chuen Neng, de Kleijn, Dominique, Sorokin, Vitaly, Sze, Siu Kwan
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Molecular biology
• Online Access: https://hdl.handle.net/10356/106377; http://hdl.handle.net/10220/38327
• Institution: Nanyang Technological University

Plasma glycoproteins and extracellular vesicles represent excellent sources of disease biomarkers, but laboratory detection of these circulating structures are limited by their relatively low abundance in complex biological fluids. Although intensive research has led to the development of effective...