Enhanced separation and characterization of deamidated peptides with RP-ERLIC-based multidimensional chromatography coupled with tandem mass spectrometry

• Main Authors: Hao, Piliang, Qian, Jingru, Dutta, Bamaprasad, Cheow, Esther Sok Hwee, Sim, Kae Hwan, Meng, Wei, Alpert, Andrew, Sze, Siu Kwan, Adav, Sunil S.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/95847; http://hdl.handle.net/10220/11322
• Institution: Nanyang Technological University

Deamidation of asparaginyl residues in proteins produces a mixture of asparaginyl, n-aspartyl, and isoaspartyl residues, which affects the proteins’ structure, function, and stability. Thus, it is important to identify and quantify the products to evaluate the effects in biological systems. It is st...