Microbubble-mediated sonoporation for highly efficient transfection of recalcitrant human B-cell lines
Sonoporation has not been widely explored as a strategy for the transfection of heterologous genes into notoriously difficult-to-transfect mammalian cell lines such as B cells. This technology utilizes ultrasound to create transient pores in the cell membrane, thus allowing the uptake of extraneous...
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Main Authors: | Yong, Charlene Li Ling, Ow, Dave Siak-Wei, Tandiono, Tandiono, Heng, Lisa Li Mei, Chan, Ken Kwok-Keung, Ohl, Claus-Dieter, Klaseboer, Evert, Ohl, Siew-Wan, Choo1, Andre Boon-Hwa |
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Other Authors: | School of Physical and Mathematical Sciences |
Format: | Article |
Language: | English |
Published: |
2015
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/106588 http://hdl.handle.net/10220/25032 http://dx.doi.org/10.1002/biot.201300507 |
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Institution: | Nanyang Technological University |
Language: | English |
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