Microbubble-mediated sonoporation for highly efficient transfection of recalcitrant human B-cell lines

• Main Authors: Yong, Charlene Li Ling, Ow, Dave Siak-Wei, Tandiono, Tandiono, Heng, Lisa Li Mei, Chan, Ken Kwok-Keung, Ohl, Claus-Dieter, Klaseboer, Evert, Ohl, Siew-Wan, Choo1, Andre Boon-Hwa
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/106588; http://hdl.handle.net/10220/25032; http://dx.doi.org/10.1002/biot.201300507
• Institution: Nanyang Technological University

Sonoporation has not been widely explored as a strategy for the transfection of heterologous genes into notoriously difficult-to-transfect mammalian cell lines such as B cells. This technology utilizes ultrasound to create transient pores in the cell membrane, thus allowing the uptake of extraneous...