The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1

The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1

We have shown that Mg/EGTA (5 mM Mg(2+) and 1.5 mM EGTA) could effectively promote the adhesion of integrin αLβ2 to its ligand ICAM-1 but could not promote that of the αMβ2 to denatured BSA. In order to determine the structural differences between αL and αM that specifically contribute to Mg/EGTA se...

Full description

Saved in:
Bibliographic Details
Main Authors: Guan, Siyu, Cheng, Ming, Law, S.K. Alex
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2015
Subjects:
DRNTU::Science::Biological sciences::Biophysics
Online Access:https://hdl.handle.net/10356/106917
http://hdl.handle.net/10220/25193
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-106917
record_format dspace
spelling sg-ntu-dr.10356-1069172023-02-28T17:00:16Z The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1 Guan, Siyu Cheng, Ming Law, S.K. Alex School of Biological Sciences DRNTU::Science::Biological sciences::Biophysics We have shown that Mg/EGTA (5 mM Mg(2+) and 1.5 mM EGTA) could effectively promote the adhesion of integrin αLβ2 to its ligand ICAM-1 but could not promote that of the αMβ2 to denatured BSA. In order to determine the structural differences between αL and αM that specifically contribute to Mg/EGTA sensitivity, a series of αL/αM chimeras were constructed. Our results showed that αLβ2 with αM calf-1 domain completely lost the response to Mg/EGTA activation. In the reverse experiment, αMβ2 would require the presence of both the αL calf-1 and calf-2 domain to initiate the Mg/EGTA sensitivity. Accepted version 2015-03-09T03:30:24Z 2019-12-06T22:21:00Z 2015-03-09T03:30:24Z 2019-12-06T22:21:00Z 2015 2015 Journal Article Guan, S., Cheng, M., & Law, S. K. A. (2015). The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1. Biochemical and biophysical research communications, 458(2), 251-255. 0006-291X https://hdl.handle.net/10356/106917 http://hdl.handle.net/10220/25193 10.1016/j.bbrc.2015.01.094 en Biochemical and biophysical research communications © 2015 Elsevier Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Biochemical and Biophysical Research Communications, Elsevier Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bbrc.2015.01.094]. 17 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Biophysics
spellingShingle DRNTU::Science::Biological sciences::Biophysics
Guan, Siyu
Cheng, Ming
Law, S.K. Alex
The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1
description We have shown that Mg/EGTA (5 mM Mg(2+) and 1.5 mM EGTA) could effectively promote the adhesion of integrin αLβ2 to its ligand ICAM-1 but could not promote that of the αMβ2 to denatured BSA. In order to determine the structural differences between αL and αM that specifically contribute to Mg/EGTA sensitivity, a series of αL/αM chimeras were constructed. Our results showed that αLβ2 with αM calf-1 domain completely lost the response to Mg/EGTA activation. In the reverse experiment, αMβ2 would require the presence of both the αL calf-1 and calf-2 domain to initiate the Mg/EGTA sensitivity.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Guan, Siyu
Cheng, Ming
Law, S.K. Alex
format Article
author Guan, Siyu
Cheng, Ming
Law, S.K. Alex
author_sort Guan, Siyu
title The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1
title_short The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1
title_full The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1
title_fullStr The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1
title_full_unstemmed The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1
title_sort integrin αl leg region controls the mg/egta mediated activation of lfa-1
publishDate 2015
url https://hdl.handle.net/10356/106917
http://hdl.handle.net/10220/25193
_version_ 1759856435216252928

Similar Items