The integrin αL leg region controls the Mg/EGTA mediated activation of LFA-1

• Main Authors: Guan, Siyu, Cheng, Ming, Law, S.K. Alex
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Biophysics
• Online Access: https://hdl.handle.net/10356/106917; http://hdl.handle.net/10220/25193
• Institution: Nanyang Technological University

We have shown that Mg/EGTA (5 mM Mg(2+) and 1.5 mM EGTA) could effectively promote the adhesion of integrin αLβ2 to its ligand ICAM-1 but could not promote that of the αMβ2 to denatured BSA. In order to determine the structural differences between αL and αM that specifically contribute to Mg/EGTA se...