Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours

Assessment of cancer predisposition syndromes (CPS) in childhood tumours is challenging to paediatric oncologists due to inconsistent recognizable clinical phenotypes and family histories, especially in cohorts with unknown prevalence of germline mutations. Screening checklists were developed to fac...

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Main Authors: Chan, Sock Hoai, Chew, Winston, Nur Diana Binte Ishak, Lim, Weng Khong, Li, Shao-Tzu, Tan, Sheng Hui, Teo, Jing Xian, Shaw, Tarryn, Chang, Kenneth, Chen, Yong, Iyer, Prasad, Tan, Enrica Ee Kar, Seng, Michaela Su-Fern, Chan, Mei Yoke, Tan, Ah Moy, Low, Sharon Yin Yee, Soh, Shui Yen, Loh, Amos Hong Pheng, Ngeow, Joanne
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/136846
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spelling sg-ntu-dr.10356-1368462020-11-01T05:20:24Z Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours Chan, Sock Hoai Chew, Winston Nur Diana Binte Ishak Lim, Weng Khong Li, Shao-Tzu Tan, Sheng Hui Teo, Jing Xian Shaw, Tarryn Chang, Kenneth Chen, Yong Iyer, Prasad Tan, Enrica Ee Kar Seng, Michaela Su-Fern Chan, Mei Yoke Tan, Ah Moy Low, Sharon Yin Yee Soh, Shui Yen Loh, Amos Hong Pheng Ngeow, Joanne Lee Kong Chian School of Medicine (LKCMedicine) Science::Medicine Cancer Genetics Paediatric Cancer Assessment of cancer predisposition syndromes (CPS) in childhood tumours is challenging to paediatric oncologists due to inconsistent recognizable clinical phenotypes and family histories, especially in cohorts with unknown prevalence of germline mutations. Screening checklists were developed to facilitate CPS detection in paediatric patients; however, their clinical value have yet been validated. Our study aims to assess the utility of clinical screening checklists validated by genetic sequencing in an Asian cohort of childhood tumours. We evaluated 102 patients under age 18 years recruited over a period of 31 months. Patient records were reviewed against two published checklists and germline mutations in 100 cancer-associated genes were profiled through a combination of whole-exome sequencing and multiplex ligation-dependent probe amplification on blood-derived genomic DNA. Pathogenic germline mutations were identified in ten (10%) patients across six known cancer predisposition genes: TP53, DICER1, NF1, FH, SDHD and VHL. Fifty-four (53%) patients screened positive on both checklists, including all ten pathogenic germline carriers. TP53 was most frequently mutated, affecting five children with adrenocortical carcinoma, sarcomas and diffuse astrocytoma. Disparity in prevalence of germline mutations across tumour types suggested variable genetic susceptibility and implied potential contribution of novel susceptibility genes. Only five (50%) children with pathogenic germline mutations had a family history of cancer. We conclude that CPS screening checklists are adequately sensitive to detect at-risk children and are relevant for clinical application. In addition, our study showed that 10% of Asian paediatric solid tumours have a heritable component, consistent with other populations. NMRC (Natl Medical Research Council, S’pore) Published version 2020-01-31T03:48:19Z 2020-01-31T03:48:19Z 2018 Journal Article Chan, S. H., Chew, W., Nur Diana Binte Ishak, Lim, W. K., Li, S.-T., Tan, S. H., . . . Ngeow, J. (2018). Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours. NPJ Genomic Medicine, 3, 30-. doi:10.1038/s41525-018-0070-7 2056-7944 https://hdl.handle.net/10356/136846 10.1038/s41525-018-0070-7 30455982 2-s2.0-85056728973 3 en NPJ genomic medicine © 2018 The Author(s). (published in partnership with the Center of Excellence in Genomic Medicine Research). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Medicine
Cancer Genetics
Paediatric Cancer
spellingShingle Science::Medicine
Cancer Genetics
Paediatric Cancer
Chan, Sock Hoai
Chew, Winston
Nur Diana Binte Ishak
Lim, Weng Khong
Li, Shao-Tzu
Tan, Sheng Hui
Teo, Jing Xian
Shaw, Tarryn
Chang, Kenneth
Chen, Yong
Iyer, Prasad
Tan, Enrica Ee Kar
Seng, Michaela Su-Fern
Chan, Mei Yoke
Tan, Ah Moy
Low, Sharon Yin Yee
Soh, Shui Yen
Loh, Amos Hong Pheng
Ngeow, Joanne
Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours
description Assessment of cancer predisposition syndromes (CPS) in childhood tumours is challenging to paediatric oncologists due to inconsistent recognizable clinical phenotypes and family histories, especially in cohorts with unknown prevalence of germline mutations. Screening checklists were developed to facilitate CPS detection in paediatric patients; however, their clinical value have yet been validated. Our study aims to assess the utility of clinical screening checklists validated by genetic sequencing in an Asian cohort of childhood tumours. We evaluated 102 patients under age 18 years recruited over a period of 31 months. Patient records were reviewed against two published checklists and germline mutations in 100 cancer-associated genes were profiled through a combination of whole-exome sequencing and multiplex ligation-dependent probe amplification on blood-derived genomic DNA. Pathogenic germline mutations were identified in ten (10%) patients across six known cancer predisposition genes: TP53, DICER1, NF1, FH, SDHD and VHL. Fifty-four (53%) patients screened positive on both checklists, including all ten pathogenic germline carriers. TP53 was most frequently mutated, affecting five children with adrenocortical carcinoma, sarcomas and diffuse astrocytoma. Disparity in prevalence of germline mutations across tumour types suggested variable genetic susceptibility and implied potential contribution of novel susceptibility genes. Only five (50%) children with pathogenic germline mutations had a family history of cancer. We conclude that CPS screening checklists are adequately sensitive to detect at-risk children and are relevant for clinical application. In addition, our study showed that 10% of Asian paediatric solid tumours have a heritable component, consistent with other populations.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Chan, Sock Hoai
Chew, Winston
Nur Diana Binte Ishak
Lim, Weng Khong
Li, Shao-Tzu
Tan, Sheng Hui
Teo, Jing Xian
Shaw, Tarryn
Chang, Kenneth
Chen, Yong
Iyer, Prasad
Tan, Enrica Ee Kar
Seng, Michaela Su-Fern
Chan, Mei Yoke
Tan, Ah Moy
Low, Sharon Yin Yee
Soh, Shui Yen
Loh, Amos Hong Pheng
Ngeow, Joanne
format Article
author Chan, Sock Hoai
Chew, Winston
Nur Diana Binte Ishak
Lim, Weng Khong
Li, Shao-Tzu
Tan, Sheng Hui
Teo, Jing Xian
Shaw, Tarryn
Chang, Kenneth
Chen, Yong
Iyer, Prasad
Tan, Enrica Ee Kar
Seng, Michaela Su-Fern
Chan, Mei Yoke
Tan, Ah Moy
Low, Sharon Yin Yee
Soh, Shui Yen
Loh, Amos Hong Pheng
Ngeow, Joanne
author_sort Chan, Sock Hoai
title Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours
title_short Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours
title_full Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours
title_fullStr Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours
title_full_unstemmed Clinical relevance of screening checklists for detecting cancer predisposition syndromes in Asian childhood tumours
title_sort clinical relevance of screening checklists for detecting cancer predisposition syndromes in asian childhood tumours
publishDate 2020
url https://hdl.handle.net/10356/136846
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