Fabrication of a biodegradable scaffold with localized response to bacterial infections

Implant-associated infections remain a significant source of morbidity in the clinic. Systemic administration of antibiotics is often ineffective, due in part to limited vascularization of the implant site. Here, we describe a physical method of incorporating antibiotics into biodegradable scaffolds...

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Main Authors: Hassanbhai, Ammar M., Lim, Jing, Wen, Feng, Chee, Heng Li, Ho, Bow, Chong, Mark S.K., Teoh, Swee Hin
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/136870
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1368702023-12-29T06:45:27Z Fabrication of a biodegradable scaffold with localized response to bacterial infections Hassanbhai, Ammar M. Lim, Jing Wen, Feng Chee, Heng Li Ho, Bow Chong, Mark S.K. Teoh, Swee Hin School of Chemical and Biomedical Engineering Engineering::Chemical engineering Polycaprolactone Cryomilling Implant-associated infections remain a significant source of morbidity in the clinic. Systemic administration of antibiotics is often ineffective, due in part to limited vascularization of the implant site. Here, we describe a physical method of incorporating antibiotics into biodegradable scaffolds. Antibiotics gentamicin sulfate (GS) and metronidazole (MZ) were cryomilled with polycaprolactone (PCL) and subsequently heat-melded. Antibiotic-loaded films were evaluated for mechanical properties, drug release characteristics, anti-microbial efficacy and cytotoxicity. Our results suggest this process to be feasible for the generation of thin film coatings with varying drug concentrations. Release profiles indicated an initial burst release for both antibiotics with a sustained release of 3 and 8 days for GS and MZ films respectively. The films inhibited bacterial growth, while viability assays suggest low mammalian cytotoxicity. Taken together, these findings establish this method as a chemical-free means to form biodegradable drug scaffolds for the tailored local delivery of antibiotics. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2020-02-04T05:00:19Z 2020-02-04T05:00:19Z 2018 Journal Article Hassanbhai, A. M., Lim, J., Wen, F., Chee, H. L., Ho, B., Chong, M. S. K., & Teoh, S. H. (2018). Fabrication of a biodegradable scaffold with localized response to bacterial infections. Merit Research Journal of Microbiology and Biological Sciences, 6(5), 054-068. 2408-7076 https://hdl.handle.net/10356/136870 5 6 054 068 en Merit Research Journal of Microbiology and Biological Sciences © 2018 MRJ. All rights reserved. This paper was published in Merit Research Journal of Microbiology and Biological Sciences and is made available with permission of MRJ. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Engineering::Chemical engineering
Polycaprolactone
Cryomilling
spellingShingle Engineering::Chemical engineering
Polycaprolactone
Cryomilling
Hassanbhai, Ammar M.
Lim, Jing
Wen, Feng
Chee, Heng Li
Ho, Bow
Chong, Mark S.K.
Teoh, Swee Hin
Fabrication of a biodegradable scaffold with localized response to bacterial infections
description Implant-associated infections remain a significant source of morbidity in the clinic. Systemic administration of antibiotics is often ineffective, due in part to limited vascularization of the implant site. Here, we describe a physical method of incorporating antibiotics into biodegradable scaffolds. Antibiotics gentamicin sulfate (GS) and metronidazole (MZ) were cryomilled with polycaprolactone (PCL) and subsequently heat-melded. Antibiotic-loaded films were evaluated for mechanical properties, drug release characteristics, anti-microbial efficacy and cytotoxicity. Our results suggest this process to be feasible for the generation of thin film coatings with varying drug concentrations. Release profiles indicated an initial burst release for both antibiotics with a sustained release of 3 and 8 days for GS and MZ films respectively. The films inhibited bacterial growth, while viability assays suggest low mammalian cytotoxicity. Taken together, these findings establish this method as a chemical-free means to form biodegradable drug scaffolds for the tailored local delivery of antibiotics.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Hassanbhai, Ammar M.
Lim, Jing
Wen, Feng
Chee, Heng Li
Ho, Bow
Chong, Mark S.K.
Teoh, Swee Hin
format Article
author Hassanbhai, Ammar M.
Lim, Jing
Wen, Feng
Chee, Heng Li
Ho, Bow
Chong, Mark S.K.
Teoh, Swee Hin
author_sort Hassanbhai, Ammar M.
title Fabrication of a biodegradable scaffold with localized response to bacterial infections
title_short Fabrication of a biodegradable scaffold with localized response to bacterial infections
title_full Fabrication of a biodegradable scaffold with localized response to bacterial infections
title_fullStr Fabrication of a biodegradable scaffold with localized response to bacterial infections
title_full_unstemmed Fabrication of a biodegradable scaffold with localized response to bacterial infections
title_sort fabrication of a biodegradable scaffold with localized response to bacterial infections
publishDate 2020
url https://hdl.handle.net/10356/136870
_version_ 1787136407625531392