Generate vascularized kidney organoids for studying kidney development

Human pluripotent stem cells (hPSCs)-derived organoids recapitulate organ architecture and development processes. Here we employ a hPSCs-derived three-dimensional (3D) kidney organoid platform to study human kidney development in vitro, which cannot be realized otherwise due to ethical consideration...

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Main Author: Zhang, Tian
Other Authors: Liang Zhao-Xun
Format: Thesis-Master by Research
Language:English
Published: Nanyang Technological University 2020
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Online Access:https://hdl.handle.net/10356/137292
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1372922023-02-28T18:49:55Z Generate vascularized kidney organoids for studying kidney development Zhang, Tian Liang Zhao-Xun Xia Yun School of Biological Sciences ZXLiang@ntu.edu.sg Science::Biological sciences Human pluripotent stem cells (hPSCs)-derived organoids recapitulate organ architecture and development processes. Here we employ a hPSCs-derived three-dimensional (3D) kidney organoid platform to study human kidney development in vitro, which cannot be realized otherwise due to ethical considerations. Using this platform, we demonstrate that a subpopulation of nephron progenitor cells (NPCs) display similar property of vascular progenitors, which eventually differentiate to renal endothelial cells in both in vitro differentiation and in vivo development. Based on this platform, we further develop an approach to modulate the differentiation of nephron compartments via manipulating metabolic pathway. Overall, hPSCs-derived kidney organoids offer new avenues for studying renal lineage specification, as well as for disease modeling in an organ-specific manner. Master of Science 2020-03-16T07:30:12Z 2020-03-16T07:30:12Z 2020 Thesis-Master by Research Zhang, T. (2020). Generate vascularized kidney organoids for studying kidney development. Master's thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/137292 10.32657/10356/137292 en This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). application/pdf Nanyang Technological University
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Biological sciences
spellingShingle Science::Biological sciences
Zhang, Tian
Generate vascularized kidney organoids for studying kidney development
description Human pluripotent stem cells (hPSCs)-derived organoids recapitulate organ architecture and development processes. Here we employ a hPSCs-derived three-dimensional (3D) kidney organoid platform to study human kidney development in vitro, which cannot be realized otherwise due to ethical considerations. Using this platform, we demonstrate that a subpopulation of nephron progenitor cells (NPCs) display similar property of vascular progenitors, which eventually differentiate to renal endothelial cells in both in vitro differentiation and in vivo development. Based on this platform, we further develop an approach to modulate the differentiation of nephron compartments via manipulating metabolic pathway. Overall, hPSCs-derived kidney organoids offer new avenues for studying renal lineage specification, as well as for disease modeling in an organ-specific manner.
author2 Liang Zhao-Xun
author_facet Liang Zhao-Xun
Zhang, Tian
format Thesis-Master by Research
author Zhang, Tian
author_sort Zhang, Tian
title Generate vascularized kidney organoids for studying kidney development
title_short Generate vascularized kidney organoids for studying kidney development
title_full Generate vascularized kidney organoids for studying kidney development
title_fullStr Generate vascularized kidney organoids for studying kidney development
title_full_unstemmed Generate vascularized kidney organoids for studying kidney development
title_sort generate vascularized kidney organoids for studying kidney development
publisher Nanyang Technological University
publishDate 2020
url https://hdl.handle.net/10356/137292
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