Mapping QTL for omega-3 content in hybrid saline tilapia

Tilapia is one of most important foodfish species. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. However, conventional breeding to i...

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Main Authors: Lin, Grace, Wang, Le, Ngoh, Si Te, Ji, Lianghui, Orbán, Laszlo, Yue, Gen Hua
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2020
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Online Access:https://hdl.handle.net/10356/137846
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1378462020-04-16T03:51:34Z Mapping QTL for omega-3 content in hybrid saline tilapia Lin, Grace Wang, Le Ngoh, Si Te Ji, Lianghui Orbán, Laszlo Yue, Gen Hua School of Biological Sciences Science::Biological sciences Tilapia Omega-3 Tilapia is one of most important foodfish species. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. However, conventional breeding to increase omega-3 content is difficult and slow. To accelerate the increase of omega-3 through marker-assisted selection (MAS), we conducted QTL mapping for fatty acid contents and profiles in a F2 family of saline tilapia generated by crossing red tilapia and Mozambique tilapia. The total omega-3 content in F2 hybrid tilapia was 2.5 ± 1.0 mg/g, higher than that (2.00 mg/g) in freshwater tilapia. Genotyping by sequencing (GBS) technology was used to discover and genotype SNP markers, and microsatellites were also genotyped. We constructed a linkage map with 784 markers (151 microsatellites and 633 SNPs). The linkage map was 2076.7 cM long and consisted of 22 linkage groups. Significant and suggestive QTL for total lipid content were mapped on six linkage groups (LG3, -4, -6, -8, -13, and -15) and explained 5.8–8.3% of the phenotypic variance. QTL for omega-3 fatty acids were located on four LGs (LG11, -18, -19, and -20) and explained 5.0 to 7.5% of the phenotypic variance. Our data suggest that the total lipid and omega-3 fatty acid content were determined by multiple genes in tilapia. The markers flanking the QTL for omega-3 fatty acids can be used in MAS to accelerate the genetic improvements of these traits in salt-tolerant tilapia. NRF (Natl Research Foundation, S’pore) 2020-04-16T03:51:34Z 2020-04-16T03:51:34Z 2018 Journal Article Lin, G., Wang, L., Ngoh, S. T., Ji, L., Orbán, L., & Yue, G. H. (2018). Mapping QTL for omega-3 content in hybrid saline tilapia. Marine biotechnology, 20(1), 10-19. doi:10.1007/s10126-017-9783-3 1436-2228 https://hdl.handle.net/10356/137846 10.1007/s10126-017-9783-3 29204906 2-s2.0-85036579209 1 20 10 19 en Marine biotechnology © 2017 Springer Science+Business Media, LLC, part of Springer Nature. All rights reserved.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Science::Biological sciences
Tilapia
Omega-3
spellingShingle Science::Biological sciences
Tilapia
Omega-3
Lin, Grace
Wang, Le
Ngoh, Si Te
Ji, Lianghui
Orbán, Laszlo
Yue, Gen Hua
Mapping QTL for omega-3 content in hybrid saline tilapia
description Tilapia is one of most important foodfish species. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. However, conventional breeding to increase omega-3 content is difficult and slow. To accelerate the increase of omega-3 through marker-assisted selection (MAS), we conducted QTL mapping for fatty acid contents and profiles in a F2 family of saline tilapia generated by crossing red tilapia and Mozambique tilapia. The total omega-3 content in F2 hybrid tilapia was 2.5 ± 1.0 mg/g, higher than that (2.00 mg/g) in freshwater tilapia. Genotyping by sequencing (GBS) technology was used to discover and genotype SNP markers, and microsatellites were also genotyped. We constructed a linkage map with 784 markers (151 microsatellites and 633 SNPs). The linkage map was 2076.7 cM long and consisted of 22 linkage groups. Significant and suggestive QTL for total lipid content were mapped on six linkage groups (LG3, -4, -6, -8, -13, and -15) and explained 5.8–8.3% of the phenotypic variance. QTL for omega-3 fatty acids were located on four LGs (LG11, -18, -19, and -20) and explained 5.0 to 7.5% of the phenotypic variance. Our data suggest that the total lipid and omega-3 fatty acid content were determined by multiple genes in tilapia. The markers flanking the QTL for omega-3 fatty acids can be used in MAS to accelerate the genetic improvements of these traits in salt-tolerant tilapia.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Lin, Grace
Wang, Le
Ngoh, Si Te
Ji, Lianghui
Orbán, Laszlo
Yue, Gen Hua
format Article
author Lin, Grace
Wang, Le
Ngoh, Si Te
Ji, Lianghui
Orbán, Laszlo
Yue, Gen Hua
author_sort Lin, Grace
title Mapping QTL for omega-3 content in hybrid saline tilapia
title_short Mapping QTL for omega-3 content in hybrid saline tilapia
title_full Mapping QTL for omega-3 content in hybrid saline tilapia
title_fullStr Mapping QTL for omega-3 content in hybrid saline tilapia
title_full_unstemmed Mapping QTL for omega-3 content in hybrid saline tilapia
title_sort mapping qtl for omega-3 content in hybrid saline tilapia
publishDate 2020
url https://hdl.handle.net/10356/137846
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