Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis
Developing optical tumor imaging probes with minimal background noise is very important for its early detection of small lesions and accurate diagnosis of cancer. To overcome the bottleneck of low signal to noise ratio and sensitivity, it needs further improvement in fluorescent probe design and und...
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sg-ntu-dr.10356-1389432020-06-01T10:26:35Z Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis Chen, Xiaohong Chen, Ziwen Hu, Benhui Cai, Pingqiang Wang, Sa Xiao, Shuzhang Wu, Yun-Long Chen, Xiaodong School of Materials Science & Engineering Innovative Center for Flexible Devices Engineering::Materials Lysosome PH Sensitive Developing optical tumor imaging probes with minimal background noise is very important for its early detection of small lesions and accurate diagnosis of cancer. To overcome the bottleneck of low signal to noise ratio and sensitivity, it needs further improvement in fluorescent probe design and understanding of tumor development process. Recent reports reveal that lysosome's acidity in cancer cells can be below 4.5 with high Na+ /H+ exchange activity, which makes it an ideal target intracellular organelle for cancer diagnosis based on the variation of pH. Herein, a boron 2-(2'-pyridyl) imidazole complex derivative (BOPIM-N) is developed, with the ability to show a pH-activatable "OFF-ON" fluorescent switch by inhibiting twisted intramolecular charge transfer upon protonation at pH 3.8-4.5, which is studied for its selective viable cancer cell imaging ability in both in vitro and in vivo experiments. Interestingly, BOPIM-N can specifically emit green fluorescence in lysosomes of cancer cells, indicating its promising cancer cell specific imaging ability. More importantly, nanoformulated BOPIM-N probes can be specifically light-ON in tumor bearing site of nude mice with resolution up to cellular level, indicating its potential application in tumor diagnosis and precision medicine. 2020-05-14T04:32:48Z 2020-05-14T04:32:48Z 2017 Journal Article Chen, X., Chen, Z., Hu, B., Cai, P., Wang, S., Xiao, S., . . . Chen, X. (2018). Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis. Small, 14(9), 1703164-. doi:10.1002/smll.201703164 1613-6810 https://hdl.handle.net/10356/138943 10.1002/smll.201703164 29265697 2-s2.0-85042675363 9 14 en Small © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved. |
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Engineering::Materials Lysosome PH Sensitive Chen, Xiaohong Chen, Ziwen Hu, Benhui Cai, Pingqiang Wang, Sa Xiao, Shuzhang Wu, Yun-Long Chen, Xiaodong Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis |
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Developing optical tumor imaging probes with minimal background noise is very important for its early detection of small lesions and accurate diagnosis of cancer. To overcome the bottleneck of low signal to noise ratio and sensitivity, it needs further improvement in fluorescent probe design and understanding of tumor development process. Recent reports reveal that lysosome's acidity in cancer cells can be below 4.5 with high Na+ /H+ exchange activity, which makes it an ideal target intracellular organelle for cancer diagnosis based on the variation of pH. Herein, a boron 2-(2'-pyridyl) imidazole complex derivative (BOPIM-N) is developed, with the ability to show a pH-activatable "OFF-ON" fluorescent switch by inhibiting twisted intramolecular charge transfer upon protonation at pH 3.8-4.5, which is studied for its selective viable cancer cell imaging ability in both in vitro and in vivo experiments. Interestingly, BOPIM-N can specifically emit green fluorescence in lysosomes of cancer cells, indicating its promising cancer cell specific imaging ability. More importantly, nanoformulated BOPIM-N probes can be specifically light-ON in tumor bearing site of nude mice with resolution up to cellular level, indicating its potential application in tumor diagnosis and precision medicine. |
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School of Materials Science & Engineering |
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School of Materials Science & Engineering Chen, Xiaohong Chen, Ziwen Hu, Benhui Cai, Pingqiang Wang, Sa Xiao, Shuzhang Wu, Yun-Long Chen, Xiaodong |
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Article |
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Chen, Xiaohong Chen, Ziwen Hu, Benhui Cai, Pingqiang Wang, Sa Xiao, Shuzhang Wu, Yun-Long Chen, Xiaodong |
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Chen, Xiaohong |
title |
Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis |
title_short |
Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis |
title_full |
Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis |
title_fullStr |
Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis |
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Synergistic lysosomal activatable polymeric nanoprobe encapsulating pH sensitive imidazole derivative for tumor diagnosis |
title_sort |
synergistic lysosomal activatable polymeric nanoprobe encapsulating ph sensitive imidazole derivative for tumor diagnosis |
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2020 |
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https://hdl.handle.net/10356/138943 |
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1681056663297064960 |