Online removal of sodium dodecyl sulfate via weak cation exchange in liquid chromatography-mass spectrometry based proteomics

Biological research often requires the use of sodium dodecyl sulfate (SDS) to solubilize protein samples; however, this detergent is not compatible with direct mass spectrometry (MS) analysis. Here, we report an online high-throughput proteomics method that permits standard in-solution digestion of...

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Bibliographic Details
Main Authors: Serra, Aida, Gallart-Palau, Xavier, Dutta, Bamaprasad, Sze, Siu Kwan
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2020
Subjects:
Online Access:https://hdl.handle.net/10356/139188
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Institution: Nanyang Technological University
Language: English