Online removal of sodium dodecyl sulfate via weak cation exchange in liquid chromatography-mass spectrometry based proteomics

• Main Authors: Serra, Aida, Gallart-Palau, Xavier, Dutta, Bamaprasad, Sze, Siu Kwan
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Biological sciences; Sodium Dodecyl Sulfate; Weak Cation Exchange
• Online Access: https://hdl.handle.net/10356/139188
• Institution: Nanyang Technological University

Biological research often requires the use of sodium dodecyl sulfate (SDS) to solubilize protein samples; however, this detergent is not compatible with direct mass spectrometry (MS) analysis. Here, we report an online high-throughput proteomics method that permits standard in-solution digestion of...