Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA

• Main Authors: Wang, Jin, Chew, Alvin Bing Liang, Lai, Yong, Dong, Hongping, Xu, Luang, Balamkundu, Seetharamsingh, Cai, Maggie Weiling, Cui, Liang, Liu, Chuan Fa, Fu, Xin-Yuan, Lin, Zhenguo, Shi, Pei-Yong, Lu, Timothy K., Luo, Dahai, Jaffrey, Samie R., Dedon, Peter C.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2020
• Subjects: Science::Medicine; RNA Characterisation and Manipulation; Mass Spectrometry-based Technique
• Online Access: https://hdl.handle.net/10356/142174
• Institution: Nanyang Technological University

Chemical modification of transcripts with 5' caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps-m7GpppN, m7GpppNm, GpppN, G...